Bardzo tanie apteki z dostawą w całej Polsce kupic viagra i ogromny wybór pigułek.
Microsoft word - microbiology_complete.doc
BL21(DE3)
F–
ompT gal dcm lon hsdSB(
rB-
mB-) λ(DE3)
- an
E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and
lacIq - Transformed plasmids containing T7 promoter driven expression are repressed until IPTG
induction of T7 RNA polymerase from a lac promoter.
BL21 (DE3) pLysS
F-
ompT gal dcm lon hsdSB(
rB-
mB-) λ(DE3) p
LysS(cmR)
- pLys plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid
- Chloramphenicol resistant
- The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which
reduces and almost eliminates expression from transformed T7 promoter containing plasmids
when not induced.
DB3.1
F-
gyrA462 endA1 glnV44 ∆(
sr1-
recA)
mcrB mrr hsdS20(
rB-,
mB-)
ara14 galK2 lacY1 proA2 rpsL20 xyl5 ∆
leu mtl1 - useful for propagating plasmids containing the ccdB gene (
gyrA462 ).
- streptomycin resistant
DH5α
F-
endA1 glnV44 thi-1
recA1 relA1 gyrA96 deoR nupG Φ80
lacZ∆
M15 ∆(
lacZYA-
argF)U169,
hsdR17(rK- mK+), λ–
- nalidixic acid resistant
DH10B (Invitrogen)
F-
endA1 recA1 galU galK deoR nupG rpsL ∆
lacX74 Φ80
lacZ∆M15
araD139 ∆(
ara,
leu)7697
mcrA ∆(
mrr-hsdRMS-mcrBC) λ-
- suitable for cloning methylated cytosine or adenine containing DNA
- Streptomycin resistant
JM109
endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ ∆(
lac-
proAB)
glnV44 e14- [F'
traD36 proAB+
lacIq lacZ∆
M15]
hsdR17(
rK-
mK+)
- Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–).
- nalidixic acid resistant
SURE (Stratagene)
endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5
uvrC e14- ∆(
mcrCB-
hsdSMR-mrr)171 F'[
proAB+
lacIq lacZ∆
M15 Tn10]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant
SURE2 (Stratagene)
endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5
uvrC e14- ∆(
mcrCB-
hsdSMR-
mrr)171 F'[
proAB+
lacIq lacZ∆
M15 Tn10
Amy CmR]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant
- chloramphenicol resistant for < 40 µg/ml, sensitive for > 100 µg/ml
XL1-Blue (Stratagene)
endA1 gyrA96 thi-1 recA1 relA1 lac glnV44 F'[ ::
Tn10
proAB+
lacIq ∆
lacZM15]
hsdR17(
rK-
mK+)
- nalidixic acid resistant
- tetracycline resistant
SW102
F-
endA1 recA1 ∆
galK deoR nupG rpsL ∆
lacX74 Φ80
lacZ∆M15
araD139 ∆(
ara,
leu)7697
mcrA ∆(
mrr-hsdRMS-mcrBC) [ λ
cI857 (
cro-bioA) <>]
- Streptomycin resistant
Source: http://francois.schweisguth.free.fr/protocols/Bacterial_strains.pdf
Prevention and Management of GastroesophagealVarices and Variceal Hemorrhage in CirrhosisGuadalupe Garcia-Tsao,1 Arun J. Sanyal,2 Norman D. Grace,3 William Carey,4 and the Practice Guidelines Committee ofthe American Association for the Study of Liver Diseases, the Practice Parameters Committeeof the American College of GastroenterologyThis guideline has been approved by the American Asso-st
Orthognathic Surgery Post Operative Instructions After you have undergone a surgical procedure to reposition your upper jaw, lower jaw, or both, attention must be directed to several aspects of post-operative care to help you make the recovery as quick and easy as possible. Since surgery produces soreness in the muscles and bones of the jaw as well as the lips, nose and other areas of the