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Conversely, injection forms, though being painful and needing help of medical personnel for application, help to quickly achieve necessary concentration of preparation in blood amoxil online Antibiotic is usually chosen in an empiric way (at random). But when choosing one is obligatory guided by definite rules.

Microsoft word - microbiology_complete.doc

BL21(DE3)

F– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3)
- an E. coli B strain with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIq
- Transformed plasmids containing T7 promoter driven expression are repressed until IPTG
induction of T7 RNA polymerase from a lac promoter.

BL21 (DE3) pLysS

F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR)
- pLys plasmid chloramphenicol resistant; grow with chloramphenicol to retain plasmid
- Chloramphenicol resistant
- The pLysS plasmid encodes T7 phage lysozyme, an inhibitor for T7 polymerase which
reduces and almost eliminates expression from transformed T7 promoter containing plasmids
when not induced.

DB3.1

F- gyrA462 endA1 glnV44 ∆(sr1-recA) mcrB mrr hsdS20(rB-, mB-) ara14 galK2 lacY1 proA2
rpsL20 xyl5 ∆leu mtl1
- useful for propagating plasmids containing the ccdB gene (gyrA462 ).
- streptomycin resistant
DH5α

F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80lacZ∆M15 ∆(lacZYA-argF)U169,
hsdR17(rK- mK+), λ–
- nalidixic acid resistant

DH10B (Invitrogen)

F- endA1 recA1 galU galK deoR nupG rpsL ∆lacX74 Φ80lacZ∆M15 araD139
∆(ara,leu)7697 mcrA ∆(mrr-hsdRMS-mcrBC) λ-
- suitable for cloning methylated cytosine or adenine containing DNA
- Streptomycin resistant

JM109

endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB+ ∆(lac-proAB) glnV44 e14- [F' traD36 proAB+
lacIq lacZ∆M15] hsdR17(rK- mK+)
- Partly restriction-deficient; good strain for cloning repetitive DNA (RecA–).
- nalidixic acid resistant

SURE (Stratagene)

endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- ∆(mcrCB-
hsdSMR-mrr)171 F'[ proAB+ lacIq lacZ∆M15 Tn10]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant

SURE2 (Stratagene)

endA1 glnV44 thi-1 gyrA96 relA1 lac recB recJ sbcC umuC::Tn5 uvrC e14- ∆(mcrCB-
hsdSMR-mrr)171 F'[ proAB+ lacIq lacZ∆M15 Tn10 Amy CmR]
- increased stability for inverted repeats and Z-DNA
- nalidixic acid resistant
- kanamycin resistant
- tetracycline resistant
- chloramphenicol resistant for < 40 µg/ml, sensitive for > 100 µg/ml

XL1-Blue (Stratagene)

endA1 gyrA96 thi-1 recA1 relA1 lac glnV44 F'[ ::Tn10 proAB+ lacIq ∆lacZM15] hsdR17(rK-
mK+)
- nalidixic acid resistant
- tetracycline resistant
SW102

F- endA1 recA1 ∆galK deoR nupG rpsL ∆lacX74 Φ80lacZ∆M15 araD139 ∆(ara,leu)7697
mcrA ∆(mrr-hsdRMS-mcrBC) [ λcI857 (cro-bioA) <>]
- Streptomycin resistant

Source: http://francois.schweisguth.free.fr/protocols/Bacterial_strains.pdf