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Hxzf.cnDetermination of resorcinol and salicylic acid content in cosmetics by reversed － phase high performance liquid chromatography GAO Xiao － xuan1 MU Min2 DONG Yin － mao1 HE Cong － fen1 ZHANG Peng － xiang1 YIN Jia － zhen1 WANG Shuo1 ZHAO Hua1 1． Beijing Technology and Business University Beijing 2． China Association of Fragrance Flavor and Cosmetic Industries Beijing Abstract A reversed － phase high performance liquid chromatography method for simultaneous determination of resorcinol and salicylic acid in cosmetics was established． The sample was analyzed on a Kromasil C column 250 mm × 4. 6 mm i． d． 5 μm with a detection UV wavelength of 275 nm． Gradient elution was carried out with mobile phase composed of methanol buffered with 0. 01 mol / L ammonium dihydro － phosphate at a flow rate of 1. 0 mL / min． Results showed that as the concentration of resorcinol and salicylic acid are in the same range of 0. 5 ～ 150 μg / mL the relationship between concentration and the corresponding peak area shows good linearity r = 0. 999 7 and 0. 999 8 respectively ． The regression equations can be expressed as A = 12 420 ρ － 6 301 and A = 15 500 ρ － 8 810 respectively and the recoveries of resorcinol and salicylic acid achieve 95. 9% ～ 103. 5% ． The RSD of the method is 0. 61% ～ 0. 88% ． Key words cosmetics high performance liquid chromatography resorcinol salicylic acid 13051236290 E － mail [email protected] rocketmail． com。 010 68984949 E － mail [email protected] btbu． edu． cn。 Standard HPLC spectrum of resorcinol 7. 7 min Precision and recoveries of resorcinol and salicylic acid HAHN S KIELHORN J KOPPENHFER J et al． Concise internation- al chemical assessment document 71 － resorcinol R ． World Health YATES R L HAVERY D C． Determination of phenol resorcinol sali- cylic acid and α － hydroxy acids in cosmetic products and salon prepa- rations J ． Journal of Cosmetics Science 1999 50 315 － 325． DEPARTMENT FOR BUSINESS ENTERPRISE ＆ REGULATORY REFORM． URN 08 /689 consultation on the consolidation of the exist- ing regulations and implementation of 3 technical directives amending directive 76 /768 / eec on the safety of cosmetic products S ． London Consumer and Competition Policy Directorate 2008． KOO J CUFFIE C A TANNER D J et al． Mometasone furoate 0． 1% － salicylic acid 5% ointment versus mometasone furoate 0． 1% ointment in the treatment of moderate － to － severe psoriasis A multicenter study J ． Clinical Therapeutics 1998 20 2 ROSSO J D． The role of topical salicylic acid in the management of psoriasis vulgaris and other localized hyperkeratotic skin disorders Evaluation of a novel cream formulation J ． Journal of the American KLIGMAN A M． The growing importance of topical retinoids in clinical dermatology A retrospective and prospective analysis J ． Journal of the American Academy of Dermatology 1998 39 Suppl 2 Results of determination for product samples MIKAMI E GOTOA T OHNOA T et al． Simultaneous analysis of dehydroacetic acid benzoic acid sorbic acid and salicylic acid in cos- metic products by solid － phase extraction and high － performance liquid chromatography J ． Journal of Pharmaceutical and Biomedical PARHAM H RAHBARA N． Solid phase extraction － spectrophotomet- ric determination of salicylic acid using magnetic iron oxide nanoparti- cles as extractor J ． Journal of Pharmaceutical and Biomedical Analy- ALGAR S O MARTOS N R DIAZ A M． Fast and single solid phase fluorescence spectroscopic batch procedure for determination in drug formulations J ． Journal of Pharmaceutical and PISTONESIA M F DI NEZIOA M S CENTURINA M E et al． Deter- mination of phenol resorcinol and hydroquinone in air samples by syn- chronous fluorescence using partial least － squares CHOCHOLOU P HOLIKA P ATINSKY D et al． A novel applica- tion of onyxTM monolithic column for simultaneous determination of sal- icylic acid and triamcinolone acetonide by sequential injection chroma- YEA G X LUA X P HAN P F et al． Application of ultrasound on J ． Chemical Engineering and Processing
C H A P T E R 4 5 Greg Hajcak ● Jonathan D. Huppert ● Edna B. Foa W H AT I S O B S E S S I V E – C O M P U L S I V E D I S O R D E R ( O C D ) ? OCD is defined by recurrent obsessions and/or compulsions that significantlyimpair functioning (American Psychiatric Association, 1994). Obsessions involveintrusive thoughts, images, or impulses that cause significant distress. Commonobsession