LABORATORIES Estrogen Receptor and Progesterone Receptor (duo pack)
Cat. No. VP-E612 Intended Use: FOR RESEARCH USE ONLY
The Estrogen Receptor and Progesterone Receptorduo pack contains:
1 x 0.5 ml Estrogen Receptor (clone 6F11)1 x 0.5 ml Progesterone Receptor (clone 1A6)
Vector Laboratories, Inc., 30 Ingold Road, Burlingame, CA 94010 U.S.A.
Tel: (650)697-3600 • Fax (650)697-0339 • Email: [email protected] • Website: www.vectorlabs.com
LABORATORIES
Antibody to Estrogen Receptor Product Specifications Description Applications: Estrogen Receptor mouse monoclonal antibody Immunohistochemistry Immunoglobulin class: Immunogen:
Prokaryotic recombinant protein representing the fulllength alpha form of the estrogen receptor molecule. Epitope: Western blotting Presentation:
Lyophilized tissue culture supernatant containing 15mM
sodium azide. Reconstitute with 0.5 ml of distilled water. Species cross-reactivity:
* Recommended dilutions using VECTASTAIN® EliteFunctional Aspects: Storage Conditions:
Estrogen receptor (ER) is expressed in a variety of
Keep unopened vial at 2 - 8 °C for 1 year. Once
different tissues but is found most prominently in the
opened it is recommended that the stock solution be
female reproductive tract. A large amount of research
aliquoted and quick frozen and stored at -20 °C. Do
has focused on the differential expression of ER in
human breast cancer. This antibody will help detectthe presence of ER in tissues and cell lines. Selected References Braidman I P, Baris C, Selby P L, et al. Preliminary report of impaired oestrogen receptor-a expression in bone, but no involvement of androgen receptor, in male idiopathic osteoporosis. Journal of Pathology. 192: 90-96 (2000). Im S, Lee E-S, Kim W, et al. Expression of progesterone receptor in human keratinocytes. Journal of Korean Medical Science. 15: 647-654 (2000). Leake R, Barnes D, Pinder S, et al. Immunohistochemical detection of steroid receptors in breast cancer: a working protocol. Journal of Clinical Pathology. 53 (8): 634-635 (2000). Kawabata K, Watanabe K, Ozaki S, et al. Utility of the paraffin-embedded section method on the detection of estrogen receptor from breast cancer tissues - comparison of the paraffin-embedded section method (6F11 and 1D5) with frozen section (H222) and dextran-coated charcoal (DCC) ones. Rinsho Byori. 47 (8): 767-773 (1999). Bevitt D J, Milton I D, Piggot N, et al. New monoclonal antibodies to oestrogen and progestrone receptors effective for paraffin section immunohis- tochemistry. Journal of Pathology. 183: 228-232 (1997). Hurlimann J, Gebhard S and Gomez F. Oestrogen receptor, progesterone receptor, pS2, ERD5, HSP27 and Cathepsin D in invasive ductal breast carcinomas. Histopathology. 23: 239-248 (1993). Snead D R J, Bell J A, Dixon A R, et al. Methodology of immunohistological detection of oestrogen receptor in human breast carcinoma in formalin- fixed, paraffin-embedded tissue: a comparison with frozen section methodology. Histopathology. 23: 233-238 (1993). Clark G M and McGuire W L. The clinical usefulness of oestrogen-receptor and other markers of hormone dependence. Proceedings of the Royal Society of Edinburgh. 95B: 145-150 (1989). Henry J A, Angus B and Horne C H W. Oestrogen receptor and oestrogen regulated proteins in human breast cancer: a review. KEIO Journal of Medicine. 38: 241-261 (1989). Shintaku P and Said J W. Detection of estrogen receptors with monoclonal antibodies in routinely processed formalin-fixed paraffin sections of breast carcinoma. American Journal of Clinical Pathology. 87: 161-167 (1987). Mason B H, Holdaway I M, Mullins P R, et al. Progesterone and oestrogen receptors as prognostic variables in breast cancer. Cancer Research. 43:2985-2990 (1983). Stefanini M, De Martino C and Zamboni L. Fixation of ejaculated spermatozoa for electron microscopy. Nature. 216: 173-174 (1967).
VP-E612 is recommended FOR PROFESSIONAL AND RESEARCH USE ONLY
Vector Laboratories, Inc., 30 Ingold Road, Burlingame, CA 94010 U.S.A.
Tel: (650)697-3600 • Fax (650)697-0339 • Email: [email protected] • Website: www.vectorlabs.com
LABORATORIES
Antibody to Progesterone Receptor Product Specifications Description Applications: Progesterone Receptor mouse monoclonal antibody Immunohistochemistry Immunoglobulin class: Immunogen:
Synthetic peptide of human progesterone receptor.
Uterus, breast carcinoma. Nuclearstaining pattern. Epitope: Western blotting Presentation:
Lyophilized tissue culture supernatant containing 15mM
sodium azide. Reconstitute with 0.5 ml of distilled water. Species cross-reactivity:
* Recommended dilutions using VECTASTAIN® Elite®ABC Kits. Storage Conditions: Keep unopened vial at 2 - 8 °C for 1 year. Once Functional Aspects:
opened it is recommended that the stock solution be
Progesterone receptor (PR) is expressed in a variety
aliquoted and quick frozen and stored at -20 °C. Do
of different tissues but is found most prominently in the
female reproductive tract. A large amount of researchhas focused on the differential expression of PR inhuman breast and uterine cancers. This antibody willhelp detect the presence of PR in tissues and cell lines. Selected References Mote P A, Johnston J F, Manninen T, et al. Detection of progesterone receptor forms A and B by immunohistochemical analysis. Journal of Clinical Pathology. 54: 624-630 (2001). Blakeman P J, Hilton P and Bulmer J N. Oestrogen and progesterone receptor expression in the female lower urinary tract, with reference to oestrogen status. BJU Int. 86 (1): 32-38 (2000). Leake R, Barnes D, Pinder S, et al. Immunohistochemical detection of steroid receptors in breast cancer: a working protocol. Journal of Clinical Pathology. 53 (8): 634-635 (2000). Clark G M and McGuire W L. The clinical usefulness of oestrogen-receptor and other markers of hormone dependence. Proceedings of the Royal Society of Edinburgh. 95B: 145-150 (1989). Giri D D, Goepel J R, Rogers K, et al. Immunohistological demonstration of progesterone receptor in breast carcinomas: correlation with radioligand binding assays and oestrogen receptor immunohistology. Journal of Clinical Pathology. 41: 444-447 (1988). Raemakers J, Beex L, Pieters G, et al. Progesterone receptor activity and the response to first endocrine therapy. European Journal of Cancer and Clinical Oncology. 23(4): 443-447 (1987). Gross G, Clark G, Chamness G, et al. Multiple progesterone receptor assays in cancer of the breast. Cancer Research. 44: 837-840 (1984). Clark G M, McGuire W L, Hubay C A, et al. Progesterone receptors as a prognostic factor in stage II breast cancer. The New England Journal of Medicine. 309(22): 1343-1347 (1983). Mason B H, Holdaway I M, Mullins P R, et al. Progesterone and oestrogen receptors as prognostic variables in breast cancer. Cancer Research. 43: 2985-2990 (1983). Stewart J, King R, Hayward J, et al. Oestrogen and progesterone receptors: correlation of response rates, sites and timing of analysis. Breast Cancer Research and Treatment. 2: 243-251 (1982). Stefanini M, De Martino C and Zamboni L. Fixation of ejaculated spermatozoa for electron microscopy. Nature. 216: 173-174 (1967).
VP-E612 is recommended FOR PROFESSIONAL AND RESEARCH USE ONLY
Vector Laboratories, Inc., 30 Ingold Road, Burlingame, CA 94010 U.S.A.
Tel: (650)697-3600 • Fax (650)697-0339 • Email: [email protected] • Website: www.vectorlabs.com
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Ultrapeel ® II plus Transderm ® Meso ® System is a powereddrug-delivery system that has been FDA approved for theFDA cleared as alternative to injections. “local administration of ionic drug solutions into the body formedical purposes and can be used as an alternative to injec-Ultrapeel® Transderm® Meso® System uses Dermoelectro-Current delivery perception protected. poration® Techn