Journal of Psychopharmacology 17(3) (2003) 342–345©2003 British Association for Psychopharmacology (ISSN 0269-8811)0269–881117:3;342–345; 033061 Case Reports Neuropsychiatric consequences (atypical psychosis and complex-partialseizures) of ecstasy use: possible evidence for toxicity–vulnerabilitypredictors and implications for preventative and clinical carePsychiatric Univ
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Azithromycin as an adjunct to scaling and root planing in the treatment of porphyromonas gingivalisassociated periodontitis: a pilot studyJ Clin Periodontol 2010; 37: 1005–1015 doi: 10.1111/j.1600-051X.2010.01607.x Alfonso Oteo1, David Herrera2,Elena Figuero1,3, Ana O’Connor3, Itziar Gonza´lez3 and Mariano Sanz21Section of Graduate Periodontology, Facultyof Odontology; 2Etiology and Therapy of Periodontal Disease (ETEP) ResearchGroup; 3Laboratory of Microbiology,Complutense University, Madrid, Spain gingivalis-associatedperiodontitis: a pilot study Oteo A, Herrera D, Figuero E, O’Connor A, Gonza´lez I, Sanz M. Azithromycin as anadjunct to scaling and root planing in the treatment of Porphyromonas gingivalis-associated periodontitis: a pilot study. J Clin Peridontol 2010; 37: 1005–1015.
AbstractObjective: To evaluate the clinical and microbiological effects of systemicazithromycin as an adjunct to scaling and root planing (SRP) in the treatment ofPorphyromonas gingivalis-associated chronic periodontitis.
Methods: Twenty-nine patients harbouring P. gingivalis were randomized into testand placebo groups. Test patients received SRP plus 500 mg of azithromycin per day(3 days), and control patients received SRP plus placebo. Clinical [plaque and bleedingindexes, probing pocket depth (PPD), clinical attachment level (CAL)] andmicrobiological data (four-sites pooled samples, processed by culture) were collectedat baseline, and 1, 3 and 6 months, post-therapy. Clinical variables were compared byANOVA, and microbiological variables by chi-square, signed-rank and Wilcoxon tests.
Results: Fifteen test and 11 placebo patients completed the study. Mean PPDdecreased 0.34 mm [95% confidence interval (CI) 0.19–0.49] in the placebo and0.80 mm (CI 0.57–1.04) in the test group after 6 months. For mean CAL gain, thecorrespondent figures were 0.29 (CI 0.08–0.49) and 0.76 (CI 0.46–1.05), respectively.
The frequency of detection of P. gingivalis decreased significantly (p40.01) in the testgroup after 1, 3 and 6 months.
Key words: azithromycin; periodontitis;P. gingivalis; microbiology; scaling and root Conclusions: Within the limitations of this study, the adjunctive use of systemic azithromycin in the treatment of P. gingivalis periodontitis demonstrated significantclinical and microbiological benefits when compared with SRP plus placebo.
porting tissues caused by specific micro- as the most relevant species, due to their pathology and their pathogenic potential.
aetiology of periodontitis is multi-factor- ial, but it is an infection and bacterial pills were provided by Pfizer, Alcobendas, microbial spectrum with in vitro activity attachment loss (Winkel et al. 1997).
elimination of these bacterial species is et al. 1987, Willians et al. 1992, Muller University Complutense, Madrid, Spain.
Winkelhoff et al. 1996, Herrera et al.
as a result of chemotactic effects exerted sites. Different in vitro (Pajukanta 1993) specific microbiological profiles, it has and in vivo (Herrera et al. 2000) studies mitans can be predictably eradicated with the adjunctive administration of amoxicil- In the last years, different studies have lin plus metronidazole (Pavicic & van is not frequent, and this bacterial species periodontitis. Conversely, P. gingivalis is et al. 2008, Pradeep et al. 2008, Yashima et al. 2009) reporting different clinical periodontitis, and it has also been found tal diseases, (vii) HIV infection, (viii) pared with other European (Sanz et al.
patients. Our hypothesis is that patients patients, the eradication of the pathogen with P. gingivalis will benefit, in terms tion of P. gingivalis from the adjunctive use of azithromycin, because this drug is selected. Clinical variables (presence of choice, due to its anti-microbial spectrum.
on P. gingivalis are not predictable.
days (Herrera et al. 2002), and the lack of et al. 1993). In addition, adverse effects, including the interference with alcoholic controlled, randomized clinical trial.
ethical committee at the Hospital Clı´n- were transferred to the microbial labora- days) and limited side effects. This short et al. 1990), and serially diluted in PBS.
they were positive for P. gingivalis.
et al. 2001). These plates were incubated for 3 days in air with 5% CO2 at 371C.
Suspected isolates were identified on the adjunctive medication was prescribed.
border and a ‘‘star’’ or ‘‘crossed cigars’’ (1 mg/l) and 5% sterile horse blood.
After 7–14 days of anaerobic incubation probe) and calibrated operator (A. O.).
in the test group received a blister con- identical in colour, form and size. Codes indole and trypsin-like activity). Counts units per millilitre of the original sam- sites selected at baseline), clinical para- bacterial species were also calculated.
effects, the overgrowth of other species, tored, especially in Dentaid-1 plates.
and inter-dental brushes (Vitis Accesssofts and Interproxs, Dentaid).
microbiological variables, especially the sion/exclusion criteria and to select the was evaluated as a control variable.
fulfilling the inclusion criteria, the pur- patient and visit and then by group.
instructions were reinforced at each visit calculated at each visit. After evaluating statistically significant (p 5 0.009).
the normality of the distribution (asses- 40 patients, 29 patients were included in baseline visit with the 1-, 3- and 6-month in the test group, with statistically sig- visit (see Fig. 1 for the flowchart of the smokers; mean age 46.6, range 38–62).
for the clinical variables. Frequencies of evaluation and the data were excluded.
square test in the inter-group assessment, and follow-up visits. Proportions of flora vided only data at the baseline visit (one antibiotic due to another disease and one visit, totaling a loss of 0.23 mm after 6 visit. An intention-to-treat analysis was Statistically significant reductions were fidence interval (CI) 0.59–0.97] after 6 The Consort E-Flowchart
Lost to fol ow-up (n=2) One took antibiotics for other One because of personal reasons One due to illness Analysis
Table 1. Demographic features of the selected sample One patient in the test group reporteddiarrhoea probably associated with the months. Out of the 13 placebo patients,13 samples were available at baseline, (significantly different as compared with baseline). No inter-group significant dif- ferences were detected at any visit or in at baseline in frequencies of detection of as inclusion criteria). A. actinomycetem- placebo and 13.3% in the test group.
rose again and were always over 80%.
Table 3. Mean values, standard error (SE) and 95% confidence intervals (CI) of changes of clinical variables between visits and inter-groupcomparison (ANCOVA, with treatment as factor and baseline value, smoking and gender as cofactors; significant cofactors are listed) CAL, clinical attachment level; PPD, probing pocket depth in mm; % PPD 1–3 mm, mean proportion of pockets in the 1–3 mm category; % PPD 4–6 mm, mean proportion of pockets in the 4–6 mm category; PlI, plaque index; BoP, bleeding on probing; RAL, relative attachment level.
values were achieved at 3 and 6 months.
p 5 0.032) of P. intermedia. P. gingivalis represented the highest proportion of the with regard to other target pathogens.
flora in both groups (21.5% in placebo and 17.7% in test samples). Higher proportions was initially observed, and then a return (po0.001), and the 6-month visit for A.
other pathogens were similar at baseline.
No significant differences were detected at baseline in proportions of flora of target Table 4a. Mean values, standard error (SE) and 95% confidence intervals (CI) of log of colony- forming units at study visits and intra-group comparison (ANOVA and multiple rank test) were also detected, specifically thoserelated with P. gingivalis. Conversely, 7.18 0.15 6.88 7.47 po0.066, baseline 7.07 0.16 6.75 7.40 p 5 0.511, relevant limitations. Only 15 patients in placebo group provided data for 6-monthvisit, and this may be considered as a Table 4b. Mean values, standard error (SE) and 95% confidence intervals (CI) of changes in log of colony-forming units between visits and inter-group comparison (ANCOVA, with treatment as factor and baseline value as cofactors; significant cofactors are listed) ences in size between both groups. Also,the effect of smoking in the results could control this variable was made by strati- failed to find significant differences in periodontal disease severity betweennon-smokers and patients who con- Table 5. Frequency of detection (in percentage) and mean proportions of flora (in percentage) of different periodontal pathogens in subgingival samples, at every study visit nez-Canut et al. 1995). Moreover,smoking was included as a cofactor in line (statistically significant, p 5 0.022) gival plaque control is an essential factor significant better clinical and microbio- logical results, despite the fact that pla- the plaque control was homogeneous inboth groups. Other baseline difference Aa, Aggregatibacter actinomycetemcomitans; Pg, Porphyromonas gingivalis; Pi, Prevotella inter-media; Tf, Tannerella forsythia; Pm, Parvimonas micra; Cr, Campylobacter rectus; Fn, Fusobac- terium nucleatum; Capno, Capnocytophaga sp.; Ec, Eikenella corrodens; Eu, Eubacterium sp.
levels in the test group (p 5 0.004).
the first study in which azithromycin has of patients with a specific microbiologi- between the test and the control groups.
(Loesche et al. 1984); presence of A.
visits on a quadrant/sextant basis within nidazole (Flemmig et al. 1998); or A.
ridement is a matter of controversy. In a results of the adjunctive use of systemic anti-microbials (Herrera et al. 2008a).
resulting in different bacterial antibiotic of clinical trials with the use of adjunc- susceptibility profiles in different Eur- versus ‘‘partial-mouth’’ (within 7 days) off et al. 2005). In order to optimize the need of periodontal surgery after SRP.
tive of the other bacteria detected. After significantly reduced in the test group at sites (Herrera et al. 2002, Guerrero et al.
positive results, and it seems reasonable not find any significant impact (Haffajee et al. 2008). With regard to T. forsythia, found in periodontal tissues. In addition, detected at any follow-up visit, as oppo- failed to find significant clinical differ- detected T. forsythia at all time periods et al. 2007). In these two studies, debri- defined in the literature; thus, it is not et al. 2002) or 3 weeks (Haffajee et al.
tions and counts of different pathogens).
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