Microsoft word - a8250 amphotericin b solubilized-说明书-可溶性两性霉素b.docx

Beijing Solarbio Science & Technology Co., Ltd. A8250 Amphotericin B solubilized 可溶性两性霉素B
产品编号： 规格
A8250-25 25mg
A8250-100 100mg
A8250-500 500mg
A8250-1000 1g
Melting Point: >170 ℃ with decomposition 1 λmax: 345, 363, 382, 406 nm (methanol)1 Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols,primarily ergosterols, of fungal 3 and bacterial cell membranes.4 After binding to sterols, it forms channels in the membranes, causing small molecules to leak out.Amphotericin B is effective against fungi and yeast. The name of the drug is derived from the amphoteric behavior of the drug, due to the carboxyl group on the main ring and a primary amino group on the Amphotericin B induces K+ leakage which is separate from its lethal action, as was demonstrated in human erythrocytes and is due to the inhibitory effect on the Na+/K+ pump.6. At sub-lethal concentrations, this drug stimulates either the activity of some membrane enzymes or cellular metabolism 3, in particular stimulation of some cells of the immune system.7 Minimum inhibitory concentrations range from 0.03-1 μg/ml for a variety of organisms including strains of Candida, Rhizopus, Asperigillus, and Coccidioides. It is inactive against bacteria, rickettsia, and viruses.Normal usage for maintenance of cell cultures is 2.5 mg/l with penicillin and streptomycin in the medium.8 For cultures already contaminated with yeast and fungus, it is recommended to use this product at 2-4 times the normal level (5-10 mg/l), without penicillin Beijing Solarbio Science & Technology Co., Ltd. and streptomycin for 2-3 subcultures. Once the infection is under control, normal maintenance levels of amphotericin B should be used. This product is for R&D use only, not for drug,household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in dimethylformamide (2-4 mg/ml) and in DMSO (30-40mg/ml). Aqueous solutions cannot be sterile Stable for 3 days in culture at 37 ℃. Stock solutions at 2-8 °C are stable for up to 1 month. For long term,storage at -20 ℃ , protected from air and light, isrecommended.1 Under these conditions the products are stable for 5 years. 1. The Merck Index, 12th ed., No. 627, (1996). 2. Clarke’s Isolation and Identification of Drugs, 2nd ed., Moffat, A. C., et al., Eds, The Pharmaceutical Press (London, GB: 1988), p. 351. 3. Bolard, J., Biochim. Biophys. Acta, 864: 257-304(1986). 4. Venegas, B., et al., Biophys. J., 85, 2323-32(2003). 5. The Pharmacological Basis of Therapeutics, 8th ed., Gilman, A. G. and Goodman, L. S., Eds., McGraw-Hill (New York, NY: 1990), p. 1165. 6. Vertut-Doi, A., et al., Biochem. Biophys. Res.Commun., 157, 692-97 (1988). 7. Sau K., et al., J. Biol. Chem., 278, 37561-68 (2003). 8. Perlman, D., Methods Enzymol., 58, 110-16 (1979).SigmaClean is a registered trademark of Sigma-Aldrich Biotechnology LP ES-S,PHC 07/07-1

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