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7801 what miniuniprep 4cFast, Convenient All-in-One
Efficient Protein Removal
Filtration for HPLC Sample Prep
for Different Sample Types
O Simple, one step, single tube method–Eliminates
centrifugation plus aspiration issues and transfer steps O Process samples 3 times faster–Purify 6 samples
in 3 minutes, 48 samples in <30 minutes O >99% protein removal for plasma samples–
% Reduction in [pr
Enables compound quantitation and extends Sample Type
O Self-sealing septa allows for repeated
The effectiveness of removing protein from different sample injections–Prevents evaporation and
types was evaluated. Plasma, serum, and whole blood (diluted1:1 in H 0) were run through the MUP-PPT procedure. The same samples were also run through a conventional spinmethodology which utilizes a 1.5 mL microfuge tube in place O Compatible with all major autosamplers
of the Mini-UniPrep, and a 5 minute centrifuge @ 10,000 xg to bring the proteins down. Supernatant was aspirated into a separate tube for analysis. Micro-BCA was used as previously Whatman Mini-UniPrep Syringeless Filters offer a highly described to quantitate proteins. All results are reported in %reduction in protein concentration.
efficient, simple alternative for removing unwanted proteinprior to HPLC/MS analysis. Ideal for performing analyticalcharacterization in drug research, the method utilizesacetonitrile precipitation and filtration by compression to Ordering Information
remove protein from plasma, serum, whole blood, and Catalog No.
Pore Size (µm)
other biological fluids. It is a single tube method that saves time and eliminates the manual transfer steps which make spin clarification problematic.
The MUP Compressor enables up to six Mini-UniPrep filter devices to be processed simultaneously. Thishandy tool will speed sample prep processing andreduce the risk of carpal tunnel syndrome.
For more information on the NEW Protein Precipitation (PPT) Protocol for Mini-UniPrep™ Syringeless Filters, or any other Whatman products call 1.800.WHATMAN
or visit our web site at www.whatman.com
Mini-UniPrep™ Protein Precipitation Protocol Add 400 µL
Add 100 µL
acetonitrile to MUP
filter plunger until
plasma to MUP
first locking ring is
compressor unit and
compress until second
lock ring is engaged
Reproducibility of Protein Removal
HPLC Quantitation of Caffeine in Plasma
% Reduction in [pr
The reproducibility of protein removal from plasma samples was Plasma samples containing varying amounts of caffeine were prepared measured by applying the MUP-PPT procedure to 40 replicates of in blinded fashion and processed using the Mini-UniPrep along with normal human plasma. Filtrate was removed from the Mini-UniPrep known plasma standards containing caffeine. After compression and dried down in a microfuge tube prior to saline resuspension filtration, the standards and “unknowns” were analyzed by HPLC and micro-BCA protein quantitation. Percent protein removal was using 10% Acn/90% H 0 mobile phase and a Partisil® 5-ODS-3 reverse calculated by comparing the protein concentrations of pre- and phase column (4.6 x 100 mm) at a flow rate of 2 mL/min. The OD275 nm caffeine peaks as measured used to determine the caffeine concen-tration of the unknown samples by comparison to the standard set.
Productivity Advantages Versus Spin Method
Single sample processing time (plasma tube ➤HPLC) ~30 seconds • close vial screw cap• place in autosampler ~45 minutes(>45 minutes if centrifugecapacity is <48 samples) 1.800.WHATMAN • 973.773.5800FAX: 973.773.0168www.whatman.com • [email protected] Copyright, Whatman Inc., 2002 Printed in USAWhatman is a trademark of Whatman International Ltd.
Mini-UniPrep™ is a trademark of Whatman Inc.
Partisil® is a registered trademark of Whatman Inc.
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