Pubblicazioni su Riviste Censite
Gennaio - Febbraio 2011
Pubblicazioni su Riviste Censite – Gennaio Febbraio 2011
1) Abeltino, M; Bonomini, S; Bolzoni, M; Storti, P; Colla, S; Todoerti, K; Agnelli, L; Neri, A;
Rizzoli, V; Giuliani, N (2011) The proapoptotic effect of zoledronic acid is independent of either
the bone microenvironment or the intrinsic resistance to bortezomib of myeloma cells and is
enhanced by the combination with arsenic trioxide
39(1):55-65 IF=3.106 [Article]
Objective Multiple myeloma (MM) cells are extremely resistant to drug induced apoptosis due to both intrinsic and bone marrow (BM) microenvironment dependent drug resistance particularly supported by bone cells Growing evidence suggest that the osteoclast inhibitor zoledronic acid (ZOL) exerts both indirect and direct anti-tumoral effects, including an in vitro proapoptotic effect on MM cells, although this property has not yet been clearly observed in MM patients Materials and Methods In this study, we attempt to better define the cytotoxic effect of ZOL on MM cells in order to identify novel drug combinations able to potentiate its proapoptotic effect Results Our data shows that ZOL at concentrations ranging from 10 to 100 mu M was able to Induce MM cell apoptosis overcoming the prosurvival effect of both stromal cells and osteo clasts and independent of the intrinsic bortezomib resistance of MM cells Interestingly, we found that the capacity of ZOL to induce apoptosis in bortezomib resistant cells was associated with a downregulation of the proapoptotic molecule myeloid cell leukemia-1 A transcriptional analysis by microarray was also performed to identify genes specifically modulated by ZOL in bortezomib resistant MM cells Finally, we show an additive effect of arsenic trioxide on apoptosis when used in combination with ZOL Conclusions Our in vitro data suggest that the use of ZOL at appropriate doses could be explored clinically in bortezomib resistant MM patients and combined with arsenic trioxide to increase its proapoptotic effect 2) Allais, G; Bussone, G; D'Andrea, G; Moschiano, F; d'Onofrio, F; Valguarnera, F; Manzoni,
; Grazzi, L; Allais, R; Benedetto, C; Acuto, G (2011) Almotriptan 12.5 mg in menstrually
related migraine: A randomized, double-blind, placebo-controlled study
31(2):144-151 IF=3.464 [Article]
3) Ampollini, L; Carbognani, P (2011) Madelung's Disease NEW ENGLAND JOURNAL
OF MEDICINE 364(5):465-465 IF=47.05 [Editorial Material]
4) Ampollini, L; Cattelani, L; Carbognani, P; Rusca, M (2011) Spontaneous abdominal-
intercostal hernia
275 IF=2.397 [Editorial Material]
Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  5) Ampollini, L; Del Rio, P; Sianesi, M; Rusca, M; Carbognani, P (2011) Transcervical
video-assisted thymectomy: preliminary results of a modified surgical approach
ARCHIVES OF SURGERY 396(2):267-271 IF=1.572 [Article]
A number of surgical approaches have been reported for thymectomy, including transsternal, transcervical, a combination of complete transsternal and transcervical, and various video-assisted thoracoscopic surgery techniques. A modified video-assisted transcervical approach to thymectomy is here described. A video-assisted total thymectomy was performed through a 30-mm cervical incision. No hyperextension of the patient's neck or sternal retractor was used. The surgical instruments utilized for the resection were the ones created for the minimally invasive video-assisted thyroidectomy. Five patients have been operated on so far. The encapsulated gland was removed without any difficulties. No complications occurred. No pain relief was administered after the first 24 h. The patients were discharged within the first two postoperative days. An improvement in clinical symptoms was registered in all patients. An advantage of this minimally invasive video-assisted transcervical approach to thymectomy is that the entire operation can be performed without neck hyperextension or permanent sternum elevation. Moreover, the surgical instruments created for minimally invasive video-assisted thyroidectomy enabled us to be very precise and to complete the resection without any postoperative morbidity. 6) Andreoli, R; Mutti, A; Goldoni, M; Manini, P; Apostoli, P; De Palma, G (2011) Reference
ranges of urinary biomarkers of oxidized guanine in (2 '-deoxy) ribonucleotides and nucleic acids
FREE RADICAL BIOLOGY AND MEDICINE 50(2):254-261 IF=6.081 [Article]
This study was aimed at defining the reference ranges for biomarkers of oxidized guanine in (2'-deoxy) ribonucleotides and nucleic acids from a large Italian sample. We recruited 300 healthy subjects (150 males; mean age 44.1 +/- 13.6 years; 26% smokers) without any known exposure to occupational oxidizing agents. They were asked to provide a spot urine sample, on which the following markers were determined by liquid chromatography-tandem mass spectrometry: 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), 8-oxo-7, 8-dihydroguanosine (8-oxoGuo), 8-oxo-7,8-dihydroguanine (8-oxoGua), and cotinine. The reference ranges, estimated as the 5th-95th percentiles of creatinine-normalized values (pmol/mu mol(creat)) were 0.7-4.2, 0.9-4.7, and 5.6-120.7 for 8-oxodGuo,8-oxoGuo, and 8-oxoGua, respectively. Oxidation biomarkers were correlated with one another (p<0.005) and with urinary creatinine (p<0.0001). Males excreted significantly higher concentrations of 8-oxoGua than females (p<0.0001). 8-OxoGua and 8-oxoGuo showed a positive association with age (p<0.001), also after stratification by gender. Multiple linear regression models including urinary creatinine concentration, age, and smoking habit as independent variables showed a significant effect of age, but not of smoking, on the levels of 8-oxoGuo in males (p<0.0001) and of both 8-oxoGuo and 8-oxoGua in females (p<0.0001). A preliminary assessment in a small group (n = 25) of patients affected by advanced non-small-cell lung cancer and receiving platinum-based chemotherapy showed significantly higher values of both 8-oxoGuo and 8-oxodGuo (p<0.0001 for both) compared to the referent population. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  7) Arcangeletti, MC; Rodighiero, I; Mirandola, P; De Conto, F; Covan, S; Germini, D; Razin,
S; Dettori, G; Chezzi, C (2011) Cell-Cycle-Dependent Localization of Human Cytomegalovirus
UL83 Phosphoprotein in the Nucleolus and Modulation of Viral Gene Expression in Human
Embryo Fibroblasts In Vitro
IF=2.935 [Article]
The nucleolus is a multifunctional nuclear compartment widely known to be involved in several cellular processes, including mRNA maturation and shuttling to cytoplasmic sites, control of the cell cycle, cell proliferation, and apoptosis; thus, it is logical that many viruses, including herpesvirus, target the nucleolus in order to exploit at least one of the above-mentioned functions. Recent studies from our group demonstrated the early accumulation of the incoming ppUL83 (pp65), the major tegument protein of human cytomegalovirus (HCMV), in the nucleolus. The obtained results also suggested that a functional relationship might exist between the nucleolar localization of pp65, rRNA synthesis, and the development of the lytic program of viral gene expression. Here we present new data which support the hypothesis of a potentially relevant role of HCMV pp65 and its nucleolar localization for the control of the cell cycle by HCMV (arrest of cell proliferation in G1-G1/S), and for the promotion of viral infection. We demonstrated that, although the incoming pp65 amount in the infected cells appears to be constant irrespective of the cell-cycle phase, its nucleolar accumulation is prominent in G1 and G1/S, but very poor in S or G2/M. This correlates with the observation that only cells in G1 and G1/S support an efficient development of the HCMV lytic cycle. We propose that HCMV pp65 might be involved in regulatory/signaling pathways related to nucleolar functions, such as the cell-cycle control. Co-immunoprecipitation experiments have permitted to identify nucleolin as one of the nucleolar partners of pp65. J. Cell. Biochem. 112: 307-317, 2011. 8) Baiardini, I; Braido, F; Ferraioli, G; Menoni, S; Bruzzone, M; Conte, ME; Gani, F; Ridolo, E;
Scordamaglia, A; Canonica, GW (2011) Pitfalls in Respiratory Allergy Management: Alexithymia
and Its Impact on Patient-Reported Outcomes
JOURNAL OF ASTHMA 48(1):25-32 IF=1.372
9) Banfi, G; Lundby, C; Robach, P; Lippi, G (2011) Seasonal variations of haematological
parameters in athletes
IF=2.047 [Review]
The influence of training and competition workloads is crucial for evaluation of longitudinal haematological data in athletes. There are only a few papers on the variation of haematological parameters during long-lasting periods and, especially, during an entire competitive season. We summarized that some haematological parameters can be influenced by long-term training and competition periods. Haemoglobin (Hb) and haematocrit (Ht) are decreased during the more intense periods of training, throughout the season. In different sport disciplines, the decline of Hb ranges from 3 to 8% during the competition season, while the range of reticulocytes (Ret%) varies from 5 to 21%. Reticulocytes are also decreased after long periods of training and competitions, but their variation is not necessarily associated with that of Hb. The qualitative variations (trend of modifications) of haematological parameters are roughly independent of the sport discipline, but quantitatively (amount of modifications) dependent on sport discipline. The modifications are more evident in cycling, running, swimming than they are in football and rugby. The variations of haematological parameters within the same sport discipline are qualitatively concordant and quantitatively different among separate but consecutive competitive seasons. These findings are described in aerobic and team sports sportsmen. The definition of reliable reference ranges in sportsmen would only be possible by Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  following the best laboratory practices. For antidoping purposes more studies investigating haematological modifications during the season are advisable. 10) Bilancia, R; Ampollini, L; Cattelani, L; Carbognani, P; Rusca, M (2011) Schwannoma
of the Cervical Vagus Nerve
[Editorial Material]
11) Brevi, BC; Toma, L; Magri, AS; Sesenna, E (2011) Use of the Mandibular Distraction
Technique to Treat Obstructive Sleep Apnea Syndrome
MAXILLOFACIAL SURGERY 69(2):566-571 IF=1.58 [Article]
12) Caffarra, P; Gardini, S; Concari, L (2011) Diagnostic Flow Chart for the Atypical
Variants of Dementia
13) Castiglioni, P; Parati, G; Brambilla, L; Brambilla, V; Gualerzi, M; Di Rienzo, M; Coruzzi, P
(2011) Detecting Sodium-Sensitivity in Hypertensive Patients Information From 24-Hour
Ambulatory Blood Pressure Monitoring
HYPERTENSION 57(2):180-185 IF=6.614 [Article]
Sodium sensitivity is an important cardiovascular risk factor for which a diagnosis requires a time-consuming protocol, the implementation of which is often challenging for patients and physicians. Our aim was to assess the reliability of an easier approach based on data from 24-hour ambulatory blood pressure monitoring performed in hypertensive subjects during daily-life conditions and habitual diet. We enrolled 46 mild to moderate hypertensive subjects who underwent 24-hour ambulatory blood pressure monitoring during usual sodium intake. Patients were divided into 3 classes of sodium sensitivity risk on the basis of ambulatory blood pressure monitoring data: low risk if dippers and a 24-hour heart rate <= 70 bpm; high risk if nondippers and a 24-hour heart rate of >70 bpm; intermediate risk with the remaining combinations (dippers with heart rate >70 bpm or nondippers with heart rate <= 70 bpm). Then patients underwent a traditional sodium sensitivity test for the dichotomous classification as sodium sensitive or sodium resistant and for evaluating the sodium sensitivity index. Prevalence of sodium-sensitive patients and mean value of sodium sensitivity index were calculated in the 3 risk classes. The sodium sensitivity index markedly and significantly increased from the low-risk to the high-risk class, being equal to 19.9 +/- 14.4, 37.8 +/- 8.3, and 68.3 +/- 17.0 mm Hg/(mol/day) in the low-risk, intermediate-risk, and high-risk classes, respectively (M +/- SEM). Also, the prevalence of sodium-sensitive patients increased significantly from the low-risk class (25%) to the intermediate-risk (40%) and high-risk (70%) classes. Thus, performance of 24-hour ambulatory blood pressure monitoring in daily-life conditions and habitual diet may give useful information on the sodium sensitivity condition of hypertensive subjects in an easier manner than with the traditional sodium sensitivity test approach. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  14) Cecchini, S; Cavazzini, E; Marchesi, F; Sarli, L; Roncoroni, L (2011) Computed
Tomography Volumetric Fat Parameters versus Body Mass Index for Predicting Short-term
Outcomes of Colon Surgery
WORLD JOURNAL OF SURGERY 35(2):415-423 IF=2.696
At present, the impact of obesity on short-term outcomes of general surgery remains controversial, especially in the field of laparoscopy. Most studies on the subject have used the body mass index (BMI) to define obesity without distinguishing between visceral and subcutaneous storage. Computed tomography (CT) volumetric analysis permits accurate evaluation of site-specific volume of adipose tissue. The purpose of this study was to compare CT volumetric fat parameters and the BMI for predicting short-term outcomes of colon surgery. A retrospective analysis was conducted of 231 consecutive patients undergoing elective colon resection, with open or laparoscopic technique, from January 2007 to April 2009. CT volumetric quantification of abdominal visceral and subcutaneous adipose tissue was performed. Intraoperative and perioperative data were collected. A total of 187 patients were enrolled. BMI showed a direct correlation with fat volumetric parameters but not with the visceral/subcutaneous fat ratio. Operating time was correlated with subcutaneous fat storage and BMI in the laparoscopic right colectomy subgroup. No associations were found with the conversion rate. Length of the hospital stay was correlated with the visceral/subcutaneous fat ratio in the laparoscopic left colectomy subgroup. Whereas the overall postoperative complication rate and mortality were not associated with fat parameters, the postoperative surgical complication rate was associated with visceral volumetric parameters in the laparoscopic left colectomy subgroup. Short-term outcomes of colon surgery are better predicted by fat volumetric parameters than by the BMI. This study has provided new elements for discussion on the impact of visceral and subcutaneous adiposity in laparoscopic and traditional colon surgery. 15) Coiro, V; Volpi, R; Stella, A; Venturi, N; Chiodera, P (2011) Stimulatory effect of
naloxone on plasma cortisol in human: Possible direct stimulatory action at the adrenal cortex
REGULATORY PEPTIDES 166(40603):1-2 IF=2.16 [Article]
The purpose of the present study was to gain a better insight in the mechanism of naloxone underlying the regulation of adrenal cortisol secretion in humans in vivo; therefore, the stimulatory effect of naloxone on cortisol secretion was assessed in a group of patients with hypothalamo-pituitary disconnection. Patients with hypothalamo-pituitary disconnection because of various pathologies (craniopharingioma, cordoma, suprasellar meningioma, or pituitary macroadenoma) participated in the study. Results: Circulating cortisol, but not adrenocorticotropin (ACTH) levels were significantly higher after naloxone administration than after saline. Conclusion: Besides the well-known hypothalamo-pituitary stimulatory action on ACTH release in normal humans, the results of the present study suggest that naloxone exerts direct effects on cortisol secretion at the adrenal gland level; another possibility is that naloxone stimulation of cortisol secretion is mediated by other factor than ACTH. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  16) Coiro, V; Volpi, R; Volta, E; Magotti, MG; Cataldo, S; Stella, A; Chiodera, P (2011)
Systemic Oxytocin Administration Modulates NPY Response to Insulin-induced Hypoglycemia in
HORMONE AND METABOLIC RESEARCH 43(2):146-147 IF=2.686 [Article]
The present study was undertaken to establish whether oxytocin (OT) is able to modify the NPY response to insulin-induced hypoglycemia in man. At 8: 00 AM of 2 different days at least 1 week apart, 10 normal men were tested with insulin (0.15 IU/kg) and with the administration of OT (infused from time -15-60 min, at a constant rate of 2 mIU/ml) or placebo. Plasma NPY concentrations rose significantly during insulin tolerance test (ITT). Oxytocin treatment significantly reduced the NPY response to hypoglycemia. The finding demonstrates for the first time in humans that the systemic administration of OT exerts an inhibitory effect on the NPY rise caused by insulin-induced hypoglycemia. 17) Corradi, D; Maestri, R; Macchi, E; Callegari, S (2011) The Atria: From Morphology to
IF=3.703 [Review]
Functional Anatomy of the Atria. The fact that some atrial and ventricular disorders (e.g., atrial fibrillation and heart failure) have a structural basis and cause atrial myocardial remodeling has led to increasing attention being paid to the atrial chambers. Furthermore, the rapid development of mapping and ablative procedures as a means of diagnosing and treating supraventricular arrhythmias has generated considerable interest in atrial gross anatomy, histology and ultrastructure. The aim of this article is to provide a comprehensive overview of the structure of the left and right atria (at macroscopic, histological and ultrastructural level) in relation to their function. In addition to analyzing normal atria, we also discuss functional anatomy in the case of atrial fibrillation and heart failure. 18) Costantino, C; Kwarecki, J; Samokhin, AV; Mautone, G; Rovati, S (2011) Diclofenac
Epolamine plus Heparin Plaster versus Diclofenac Epolamine Plaster in Mild to Moderate Ankle
Sprain A Randomized, Double-Blind, Parallel-Group, Placebo-Controlled, Multicentre, Phase III
CLINICAL DRUG INVESTIGATION 31(1):15-26 IF=1.414 [Article]
Background: In general sports, ankle sprain is the most frequently reported ankle injury and can cause chronic lateral ankle pain and tenderness. Treatment with NSAIDs is preferred, and several topical NSAID formulations are now available, helping to avoid the systemic adverse events typically associated with oral preparations. Objective: To compare the efficacy and tolerability of a newly developed fixed-dose diclofenac epolamine (diclofenac hydroxyethylpyrrolidine, DHEP)/heparin plaster (Flectoparin (R) Tissugel) with that of a DHEP (Flector EP Tissugel (R)) or placebo plaster in the treatment of mild to moderate ankle sprain in adults. Methods: This was a randomized, double-blind, parallel-group, placebo-controlled, multicentre, phase III study conducted in the emergency medical centres of hospitals or private clinics in Europe. Outpatients aged 18-65 years who had suffered an acute ankle sprain (O'Donoghue grade I or II in severity, with external lateral ligament involvement) within the previous 48 hours and had peri-malleolar oedema were eligible for inclusion. A total of 430 patients were randomized to receive a DHEP/heparin 1.3%/5600 IU (n = 142), DHEP 1.3% (n = 146) or placebo (n = 142) plaster, applied once daily to the injured ankle for a total of 7 days. The primary endpoint was the mean change from baseline in pain on movement on day 3, as measured by a visual analogue scale (VAS). Results: The DHEP/heparin plaster was associated with a significantly (p = 0.002) greater mean reduction from baseline in pain on Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  movement after 3 days of treatment than the DHEP plaster (-24.2 vs -18.8 mm VAS), with each active treatment providing significantly (p <= 0.005) greater pain relief than placebo (-13.7 mm VAS). Both DHEP/heparin and DHEP were also effective in relieving other measures of pain, with DHEP/heparin recipients experiencing significantly less daily pain while leaning on the injured limb than DHEP recipients (p < 0.001). In addition, oedema was reduced to a significantly greater extent with DHEP/heparin than with placebo (day 7 only; p = 0.012). The DHEP/heparin plaster and DHEP plaster were both well tolerated, with adverse event profiles similar to that of placebo. Local adverse events were infrequent and generally mild in severity and there were no systemic adverse effects. Conclusion: The fixed-dose DHEP/heparin plaster is effective and has advantages over the DHEP plaster in relieving pain, and possibly also swelling, associated with mild to moderate acute ankle sprains with oedema in adults. 19) Costi, R; Di Mauro, D; Veronesi, L; Ardizzoni, A; Salcuni, P; Roncoroni, L; Sarli, L;
Violi, V
(2011) Elective palliative resection of incurable stage IV colorectal cancer: Who really
benefits from it?
SURGERY TODAY 41(2):222-229 IF=1.05 [Article]
Despite the encouraging results of chemotherapy in patients affected by incurable colorectal cancer (CRC), surgical resection of a primitive tumor is still a common approach worldwide. The identification of prognostic factors related to short survival (< 6 months) may allow excluding from resective surgery those who may not benefit from it. A retrospective analysis was performed of 15 variables in a population of 71 patients undergoing nonemergency palliative primary resections of incurable CRC, including patients' demographics and clinical/histopathological characteristics of the tumor. No variables were related to perioperative mortality (8.5% overall). A multivariate analysis revealed that older age (a parts per thousand yen80 years) and metastasis to more than 25% of the lymph nodes were associated with survival (4 and 6 months, respectively). Mucoid adenocarcinoma therefore tends to be associated with the prognosis (P = 0.070). An elderly age tends to be a contraindication to an elective primary tumor resection in patients affected by incurable CRC. Massive lymph node involvement and mucoid adenocarcinoma should also be considered before planning major colonic surgery. 20) Dadomo, H; Volpi, R; Ferrari, M; Vignali, A; Bartolomucci, A; Palanza, P; Parmigiani, S
(2011) Sildenafil counteracts the inhibitory effect of social subordination on competitive
aggression and sexual motivation in male mice
216(1):193-199 IF=3.22 [Article]
The effects of chronic intra-peritoneal administration of 10 mg/kg (t.i.w., for 5 weeks) of sildenafil on competitive aggression, sexual behaviour and body weight gain was tested in CD1 subordinate male mice in two experimental contexts: 1) "low levels of aggression", i.e. housing in dyads of siblings 2) "high levels of aggression", i.e. exposure to a model of chronic psychosocial stress with an unfamiliar mice. Subordinate mice in both experimental contexts were injected with sildenafil or saline. After 2 weeks of sildenafil administration, a subgroup of subordinates exposed to "high levels of aggression" began to counterattack their dominant counterparts at higher rates than saline-injected subordinates. This effect was essentially similar but faster in subordinates subjected to "low levels of aggression". As far as sexual behaviour is concerned, in both experimental contexts, sildenafil-injected subordinated mice showed significant lower latencies to mount a proceptive female when compared to saline-injected subjects. Furthermore, in the "high levels of aggression" context, Sildenafil reduced stress-induced body weight gain. Sildenafil showed no effects in individually housed males serving as controls. In conclusion, chronic Sildenafil treatment Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  counteracts the inhibitory effects of social subordination on male competitive aggression, sexual behaviour and body weight gain. Overall our data suggests that sildenafil could be acting in the central nervous system to modulate sexual and agonistic motivation. (C) 2010 Elsevier B.V. All rights reserved. 21) D'Aloja, C; D'Aloja, E; Santi, E; Franchini, M (2011) The use of fresh-frozen bone in oral
surgery: a clinical study of 14 consecutive cases
Background. Although autologous bone is considered the gold standard among the grafting materials used in implant therapy, it does have a number of drawbacks, in particular morbidity at the site of donation and the limited amount of bone available. To overcome these limitations a number of alternative bone materials have been employed in the last few years. In this study we report the results of the use of homologous fresh-frozen bone from a tissue bank in patients undergoing reconstruction of bone defects in the oral cavity. Material and methods. Between June 2004 and October 2008, 14 consecutive patients underwent bone reconstruction with fresh-frozen bone from a tissue bank. Four to eight months after surgery, implants were placed in the newly formed bone. Results. No problems were recorded during the post-operative course. In all cases treatment was successful and osteointegrated implants were placed in the newly formed bone after 4-8 months. All implants showed good osteointegration (100% overall success rate, mean follow-up 20 months), allowing loading with a fixed cemented prosthesis. Conclusions. Our results support the previous findings that homologous fresh-frozen bone can be considered a valid alternative to autologous bone for the reconstruction of bone defects in the oral cavity in patients undergoing implant therapy. 22) De Caterina, R; Talmud, PJ; Merlini, PA; Foco, L; Pastorino, R; Altshuler, D; Mauri, F;
Peyvandi, F; Lina, D; Kathiresan, S; Bernardinelli, L; Ardissino, D (2011) Strong association of
the APOA5-1131T > C gene variant and early-onset acute myocardial infarction
ATHEROSCLEROSIS 214(2):397-403 IF=4.522 [Article]
Background: Epidemiological studies support the role for a strong genetic component in the occurrence of early-onset myocardial infarction (MI), although the specific genetic variants responsible for familial clustering remain largely unknown. Methods: The Italian study of early-onset MI is a nationwide case-control study involving 1864 case patients <45 years old who were hospitalized for a first MI, and age/sex/place of origin-matched controls (n = 1864). We investigated the association between early-onset MI, lipid levels and 20 single nucleotide polymorphisms (SNPs) in the candidate genes ADIPOQ, APOA5, ALOX5AP, CYBA, IL6, LPL, PECAM1, PLA2G2A and PLA2G7, chosen because of previously reported associations with Coronary Heart Disease (CHD) or with CHD risk factors. Results: Of all the SNPs investigated, APOA5-1131T>C [(rs662799), minor allele frequency 0.084 (95% confidence interval (CI) 0.07-0.09)] alone showed a statistically significant association with risk of early-onset MI (p = 6.7 x 10(-5)), after Bonferroni correction, with a per C allele odds ratio of 1.44 (95% CI 1.23-1.69). In controls, APOA5-1131T>C was significantly associated with raised plasma triglyceride levels (p = 0.001), compared with non-carriers, the per C allele increase being 11.4% (95% CI 4-19%), equivalent to 0.15 mmol/L (95% CI 0.11-0.20 mmol/L). In cases, the association with early MI risk remained statistically significant after adjustment for triglycerides (p = 0.006). Conclusions: The APOA5-1131C allele, associated with higher fasting triglyceride levels, strongly affects the risk for early-onset MI, even after adjusting for triglycerides. This raises the possibility that APOA5-1131T>C may affect the risk of early MI over and above effects mediated by triglycerides. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  23) De Conto, F; Covan, S; Arcangeletti, MC; Orlandini, G; Gatti, R; Dettori, G; Chezzi, C
(2011) Differential infectious entry of human influenza A/NWS/33 virus (H1N1) in mammalian
kidney cells
VIRUS RESEARCH 155(1):221-230 IF=2.563 [Article]
In this report we focused our interest on the early events of the replication cycle of NWS/33 human influenza A (NWS) virus in MDCK (canine), LLC-MK2 (simian), and NSK (swine) kidney cells, with different susceptibility upon infection. We have previously demonstrated that actin organization induces restriction to viral replication during the early stages of NWS virus infection in simian kidney cells. To explore how cell endocytic mechanisms are hijacked by NWS virus and may modulate the outcome of viral infection, the effect of drugs affecting selectively the entry via clathrin-coated pits, caveolar/raft-dependent endocytosis and macropinocytosis was analyzed. Results point to critical differences in terms of internalization pathways exploited by NWS virus to enter the examined cell models. Moreover, we show that some ways of entry do not allow an effective virus internalization, depending on the cell type. Understanding how specific cell functions/components may regulate early phases of viral replication allows us to deepen our knowledge on influenza virus infection and provides new insights for anti-viral researches. 24) De Filippo, M; Corsi, A; Evaristi, L; Bertoldi, C; Sverzellati, N; Averna, R; Crotti, P; Bini,
G; Tamburrini, O; Zompatori, M; Rossi, C (2011) Critical issues in radiology requests and
RADIOLOGIA MEDICA 116(1):152-162 IF=1.454 [Article]
This study evaluated the appropriateness and accuracy of 500 radiology requests and their matched reports in order to identify recurring errors in both areas. A randomly chosen sample consisting of 167 computed tomography (CT), 166 ultrasonography (US) and 167 radiographic examinations were collected and analysed according to national referral guidelines and to the principles of justification and optimisation (Law no. 187/2000). We identified a high rate of inappropriate requests (27.6%) and requests lacking a clinical question (22%). There was good precision in the anamnestic data (80.6%) and in the formulation of the diagnostic question (76.8%). Almost all requests were handwritten, and 12.5% lacked the referring physician's stamp and/or signature. No report mentioned the clinical information received or the equipment used. The use of contrast medium was always reported. Conclusions were reported in 9.8% of these reports. When further investigation would have been necessary, the radiologist omitted to report this in 60% of cases. Some important weaknesses emerged, especially regarding requests for radiological examinations (22% lacked the clinical question, 27.6% were inappropriate), potentially limiting the effectiveness of the diagnostic process and leading to negative effects on the correct risk management process. There emerges a need for better collaboration between clinicians and radiologists. 25) de Troia, A; Tecchio, T; Azzarone, M; Biasi, L; Piazza, P; Salcuni, PF (2011)
Endovascular Treatment of an Innominate Artery Iatrogenic Pseudoaneurysm Following
Subclavian Vein Catheterization
82 IF=0 [Article]
Central venous catheterization is routinely required in a wide range of clinical situations such as hemodynamic monitoring, delivery of blood products and drugs, hemodialysis, total parental nutrition, and management of perioperative fluids. Pseudoaneurysms account for 4% of all innominate artery aneurysms. Clinical presentation may include airway and neurological compression, chest pain, mediastinal syndrome, Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  upper limb ischemia, and hemodynamic instability. We report what we believe is the first documented case of a successful endovascular stent-graft repair of an innominate artery iatrogenic pseudoaneurysm. 26) Di Perna, C; Franchini, M; Riccardi, F; Rivolta, GF; Angeri, F; Tagliaferri, A (2011)
Association between haemophilia and inherited thrombophilia: a single centre survey
HAEMOPHILIA 17(1):161-162 IF=2.505 [Letter]
27) Favaloro, EJ; Lippi, G (2011) Coagulation update: What's new in hemostasis testing?
THROMBOSIS RESEARCH 127():0-0 IF=2.406 [Article]
The current report provides a personal perspective summarising some interesting recent developments in hemostasis, as well as providing a brief glimpse into some possible imminent changes to come. We briefly review routine coagulation tests, and what changes may take place related to the new emerging anticoagulants. We also briefly review the old and new global tests of hemostasis, including thrombin generation and thromboelastography. Also briefly discussed within the diagnostics of bleeding and thrombotic disorders are the role of microparticles, the rise and fall of thrombophilia testing, the 'disappearance' of fibrinolysis pathway tests, and the absence of tests related to the endothelium, in part reflecting upon Virchow's triad. 28) Filonzi, L; Magnani, C; Marzano, FN (2011) Confirmed association between monoamine
oxidase A molecular polymorphisms and Sudden Infant Death Syndrome
12(1):91-92 IF=3.486 [Letter]
29) Fogagnolo, P; Sangermani, C; Oddone, F; Frezzotti, P; Iester, M; Figus, M; Ferreras, A;
Romano, S; Gandolfi, S; Centofanti, M; Rossetti, L; Orzalesi, N (2011) Long-term perimetric
fluctuation in patients with different stages of glaucoma
OPHTHALMOLOGY 95(2):189-193 IF=2.917 [Article]
30) Franchini, M (2011) Hepcidin: the main regulator of iron homeostasis CLINICAL
CHEMISTRY AND LABORATORY MEDICINE 49(2):165-166 IF=1.886 [Editorial
31) Franchini, M; Targher, G; Lippi, G (2011) Safety of Recombinant Activated Factor VII in
Randomized Clinical Trials
IF=47.05 [Letter]
32) Franchini, M; Vescovi, PP; Manzato, F; Favaloro, EJ (2011) Hormones, Endocrine
Disorders, and Hemostasis PREFACE
37(1):3-5 IF=3.214 [Editorial Material]
33) Fusco, C; Ucchino, V; Barbon, G; Bonini, E; Mostacciuolo, ML; Frattini, D; Pisani, F; Della
Giustina, E (2011) The Homozygous Ganglioside-Induced Differentiation-Associated Protein 1
Mutation c.373C > T Causes a Very Early-Onset Neuropathy: Case Report and Literature Review
JOURNAL OF CHILD NEUROLOGY 26(1):49-57 IF=1.592 [Review]
Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  34) Gaibazzi, N; Reverberi, C; Badano, L (2011) Usefulness of Contrast Stress-
Echocardiography or Exercise-Electrocardiography to Predict Long-Term Acute Coronary
Syndromes in Patients Presenting With Chest Pain Without Electrocardiographic Abnormalities or
12-Hour Troponin Elevation
IF=3.575 [Article]
The evaluation of patients presenting to the hospital with a recent episode of chest pain suggestive of myocardial ischemia, nondiagnostic electrocardiographic findings, and normal 12-hour cardiac troponin levels remains a challenge for the clinician. We selected 1,081 consecutive patients who presented to the emergency department during 2008 for a chest pain complaint of suspected cardiac origin without significant electrocardiographic abnormalities or troponin elevation. These patients underwent either contrast-enhanced stress-echocardiography with myocardial perfusion imaging or exercise-electrocardiography within 5 days of the index admission. We analyzed their 1-year cardiac outcome (i.e., unstable angina, myocardial infarction, or cardiac death). A post test likelihood of cardiac events was determined on the basis of the results of the provocative testing. Significantly better event-free survival (log-rank p<0.0001) was found for both hard (cardiac death and nonfatal myocardial infarction) and combined (acute coronary syndrome) end points in patients with normal contrast-enhanced stress-echocardiographic findings. However, this was not the case for patients in the exercise-electrocardiographic group, for whom event-free survival was not significantly different among the 3 possible result categories (normal, indeterminate, and abnormal test findings; log-rank p = NS). In conclusion, inducible ischemia detected by contrast-enhanced stress-echocardiography predicted the 1-year incidence of acute coronary syndrome (11.3% for positive vs 0.8% for negative results). However, this was not the case for exercise-electrocardiography, with a 2.7%, 2.3%, and 2.9% 1-year incidence of acute coronary syndromes for positive, negative, and indeterminate results, respectively. (C) 2011 Elsevier Inc. All rights reserved. 35) Gatta, L; Di Mario, F; Vaira, D; Franze, A; Rugge, M; Pilotto, A; Lucarini, P; Lera, M;
Fiorini, G; Castelli, V; Kajo, E; Scarpignato, C (2011) Helicobacter pylori eradication: are we
really all equal? A controlled study in native and immigrant population
EMERGENCY MEDICINE 6(1):35-39 IF=2.371 [Article]
Italy's shift to a tertiary economy has modified the working market, concentrating demand also on unqualified one, which includes most immigrants. It is also well established that low socio-economical conditions are associated with an increased prevalence of H. pylori infection. The aims of this study were to compare: (1) the efficacy of a 7 days triple therapy in immigrant and in Italian patients; (2) the prevalence of PUD between these two groups of patients. A total of 116 consecutive immigrant and 112 Italian H. pylori infected patients were recruited between 2007 and 2008. Patients underwent C-13-UBT, endoscopy with biopsies, and were offered a 7-day triple therapy. Eradication rate (ER) was assessed 8 weeks after the end of the treatment using C-13-UBT. The two populations differed for median age (p < 0.01), prevalence of PUD (p < 0.01), and smoking status (p < 0.01). The ER according to the ITT analysis was 70% for Italian and 48.3% for immigrant (p < 0.01). Multivariate analysis including country of origin, sex, age, PUD, smoking, and alcohol status found that immigrant had an adjusted OR for not eradicating of 2.14 (p = 0.03). In immigrant patients resident in Italy, performance of triple therapy was lower than expected. Further studies are demanded to confirm and clarify these intriguing results. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  36) Giordano, G; D'Adda, T; Dal Bello, B; Brigati, F; Bersiga, A; Campanini, N; Berretta, R;
Rocco, A; Merisio, C (2011) Clinicopathologic Implications of the Epidermal Growth Factor
Receptor, Cyclooxygenase 2 Expression, and Human Papillomavirus Status in Squamous Cell
Carcinoma of the Uterine Cervix in the Elderly
GYNECOLOGICAL CANCER 21(2):337-348 IF=2.179 [Article]
Objectives: To find information on invasive squamous cervical carcinoma in the elderly, 110 invasive squamous cervical carcinomas obtained from 2 groups of patients (aged <60 and 960 years) were analyzed for human papillomavirus (HPV) status by polymerase chain reaction study, for immunohistochemical epidermal growth factor receptor (EGFR), cyclooxygenase 2 (Cox-2) expression, and clinicopathologic features. Methods: The HPV status and the expression of Cox-2 and EGFR in the younger and older women were compared and correlated with the grading, staging neoplasm, and lymph nodal status, using Fisher test and Spearman nonparametric correlation test. Overall survival curves were drawn using Kaplan-Meier estimates and were compared using log-rank tests in the whole series of 110 patients. Multinomial logistic regression was also used. Results and Conclusions: The number of neoplasms with higher staging was significantly greater than those in the younger women (P = 0.04). The mortality was higher in the older group than in the younger patients (P = 0.006). In the elderly, the presence of HPV DNA in 65% of cases, and in the absence of sexual activity, could be due to reactivation of latent HPV infection, which might be due to an impairment of host immunologic response. The overexpression of Cox-2 in a number of cases was significantly higher in the older group than in the younger group (P = 0.032, Fisher exact test), but this immunoreactivity is not related to the staging, grading, EGFR expression, or to the presence of HPV. The simultaneous expression of Cox-2 and EGFR had a poor prognostic significance, showing lower survival rates than cases without this immunoreactivity (P = 0.002), on univariate analysis. On multivariate analysis, Cox-2 and EGFR immunopositivity did not reveal any correlation between these markers and prognosis probably because the number of cases considered was not particularly high. 37) Lagging, M; Askarieh, G; Negro, F; Bibert, S; Soderholm, J; Westin, J; Lindh, M; Romero,
A; Missale, G; Ferrari, C
; Neumann, AU; Pawlotsky, JM; Haagmans, BL; Zeuzem, S; Bochud,
PY; Hellstrand, K (2011) Response Prediction in Chronic Hepatitis C by Assessment of IP-10 and
IL28B-Related Single Nucleotide Polymorphisms
PLOS ONE 6(2):- IF=4.351 [Article]
38) Lippi, G; Cervellin, G (2011) Letter by Lippi and Cervellin Regarding Article, "High-
Sensitivity Troponin T Concentrations in Acute Chest Pain Patients Evaluated With Cardiac
Computed Tomography"
CIRCULATION 123(1):0-0 IF=14.816 [Letter]
39) Lippi, G; Cervellin, G; Banfi, G; Plebani, M (2011) Cardiac troponins and physical
exercise. Its time to make a point
BIOCHEMIA MEDICA 21(1):55-62 IF=0.66 [Review]
The timely diagnosis of acute coronary syndrome (ACS), in particular myocardial infarction (MI), is still one of the most challenging issues in medicine. The introduction into routine laboratory practice of assays for measuring the cardiospecific troponins has dramatically revolutionized the diagnostic approach and the recent development of methods with improved analytical sensibility (i.e., highly sensitivity [HS] assays), has further contributed to improve the negative predictive value of troponin testing but, contextually, has substantially lowered the clinical specificity of these markers. In particular, clinical studies have demonstrated the existence of an exercise-related increase of HS-troponins, with measurable values detectable in up to 94% of athletes undergoing endurance sports. This measurable amount of troponin in blood would mirror an increased membrane permeability and early troponin release rather than reflecting a Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  clinically threatening myocardial injury. As such, the measurable amount of cardiac troponins as assessed with the novel HS assays requires major clinical focus (i.e., serial measurement of cardiac biomarkers, detailed clinical history-taking, integration with ECG and imaging findings) to prevent misdiagnosis of ACS and/or MI in otherwise healthy persons. 40) Lippi, G; Cervellin, G; Franchini, M (2011) Tranexamic Acid Treatment for Heavy
Menstrual Bleeding: A Randomized Controlled Trial
117(1):176-176 IF=4.357 [Letter]
41) Lippi, G; Franchini, M (2011) Vitamin K in neonates: facts and myths BLOOD
TRANSFUSION 9(1):4-9 IF=0 [Review]
42) Lippi, G; Franchini, M (2011) Troubleshooting anaemia in the elderly In reply BLOOD
TRANSFUSION 9(1):110-111 IF=0 [Letter]
43) Lippi, G; Franchini, M (2011) Home Monitoring of Warfarin Effects NEW ENGLAND
JOURNAL OF MEDICINE 364(4):378-378 IF=47.05 [Letter]
44) Lippi, G; Franchini, M; Banfi, G (2011) Normobaric hypoxia and sports: the debate
45) Lippi, G; Franchini, M; Targher, G (2011) Detection of chronic kidney disease in
hospitalized patients: Is one estimating glomerular filtration rate equation better than another?
46) Lippi, G; Montagnana, M; Danese, E; Favaloro, EJ; Franchini, M (2011) Glycoprotein
IIb/IIIa inhibitors: an update on the mechanism of action and use of functional testing methods to
assess antiplatelet efficacy
BIOMARKERS IN MEDICINE 5(1):63-70 IF=0.861 [Review]
The human glycoprotein (GP)IIb/IIIa belongs to a large family of cation-dependent adhesion molecules known as integrins, which share a common heterodimeric structure. The primary function of GPIIb/IIIa is to aid platelet aggregation by transmitting bidirectional signals across the plasma membrane. Since the GPIIb/IIIa receptor is among the key integrins involved in platelet aggregation and, therefore, thrombus formation, the development of GPIlb/Illa antagonists (e.g., abciximab, eptifibatide and tirofiban) has become an attractive strategy for antiplatelet therapy with an expected strong and specific effect. All three drugs are administered intravenously, and large-scale clinical trials have demonstrated a clear clinical benefit and good safety profile in high-risk patients, especially those undergoing percutaneous coronary intervention. However, the adverse events related to thrombosis or bleeding are still reported in patients undergoing therapy with GPIlb/Illa antagonists and reflect a variable interindividual responsiveness. Therefore, some form of laboratory monitoring is required to optimize the effects of a drug or to indicate that it needs replacing with other antithrombotic agents, as well as for identifying and enhancing the platelet inhibition in this subgroup of patients to improve the clinical outcome and reduce bleeding complications. As such, the aim of this article is to provide an update on the mechanism of action and use of functional testing methods to assess antiplatelet efficacy in patients undergoing therapy with GPIIb/IIIa antagonists. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  47) Lippi, G; Montagnana, M; Franchini, M (2011) Ex-vivo red blood cells generation: A step
ahead in transfusion medicine?
19 IF=1.385 [Review]
Blood transfusion and especially red blood cell (RBC) transfusion is an essential therapeutic act because it might be life-saving in several situations such as massive bleeding or as prolonged quality-of-life therapies in patients with chronic anemic disorders. Although the need for blood is thereby widespread, there is however a major imbalance between demand and supply of donors, so that there is a mounting research to develop suitable surrogates for human donated blood. Functional RBCs have already been generated from a variety of cellular progenitors (i.e., somatic stem cells, human embryonic stem cells and induced pluripotent stem cells) and synthetic biomaterials. Although these types of "artificial blood" carry several advantages over donated blood, including larger supply, lower risk of blood-borne pathogens transmission, no risk of immune incompatibility using group O, RhD-negative RBCs, and extended survival of stored RBCs, their efficacy has not been extensively tested thus far in clinical trials. Therefore, while it seems premature at this point in time to conclude that ex-vivo manufacturing of blood might be the definitive solution to the current shortage of blood supply, it represents however a valuable starting point for translational research in transfusion medicine. 48) Lippi, G; Plebani, M (2011) Pharmacogenomics of alcohol metabolism: implications for
legal testing
IF=1.886 [Editorial Material]
49) Lippi, G; Plebani, M (2011) Reduction of unsuitable specimens: A more radical and
comprehensive approach is needed
CLINICA CHIMICA ACTA 412(40636):400-400
IF=2.535 [Letter]
50) Maffei, E; Seitun, S; Martini, C; Aldrovandi, A; Arcadi, T; Clemente, A; Messalli, G;
Malago, R; Weustink, A; Mollet, N; Nieman, K; Ardissino, D; de Feyter, P; Krestin, G;
Cademartiri, F (2011) Prognostic value of CT coronary angiography: focus on obstructive vs.
nonobstructive disease and on the presence of left main disease
116(1):15-31 IF=1.454 [Article]
The authors investigated the prognostic value of computed tomography coronary angiography (CTCA) for major adverse cardiac events (MACE) in patients with suspected or known coronary artery disease (CAD), with particular focus on left main (LM) disease and obstructive vs. nonobstructive disease. A total of 727 consecutive patients (485 men, age 62 +/- 11years) with suspected (514; 70.1%) or known (213; 29.9%) CAD underwent CTCA. Patients were followed up for the occurrence of MACE (i.e. cardiac death, nonfatal myocardial infarction, unstable angina, percutaneous/surgical revascularisation). A total of 117 MACE [five cardiac deaths, 11 acute myocardial infarctions (AMI), five unstable angina, 86 percutaneous coronary interventions, ten coronary artery bypass grafts] occurred during a mean follow-up of 20 months. Severity and extension of CAD was associated with a progressively worse prognosis. The event rate was 0% among patients with normal coronary arteries at CTCA. The presence of LM disease was not associated with a worse prognosis either in patients with no history of CAD or in those with a history of CAD. At multivariate analysis, presence of obstructive CAD and diabetes were the only independent predictors of MACE. Evaluation of atherosclerotic burden by CTCA provides an independent prognostic value for prediction of MACE. Patients with normal CTCA findings have an excellent prognosis at follow-up. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  51) Manari, A; Ortolani, P; Guastaroba, P; Marzaroli, P; Menozzi, M; Magnavacchi, P; Varani, E;
Vignali, L; Campo, G; Marzocchi, A (2011) Long-term outcomes with cobalt-chromium bare-
metal vs. drug-eluting stents: the REgistro regionale AngiopLastiche dell'Emilia-Romagna registry
52) Marchetti, F; Maestro, A; Rovere, F; Zanon, D; Arrighini, A; Bertolani, P; Biban, P; Da Dalt,
L; Di Pietro, P; Renna, S; Guala, A; Mannelli, F; Pazzaglia, A; Messi, G; Perri, F; Reale, A;
Urbino, AF; Valletta, E; Vitale, A; Zangardi, T; Tondelli, MT; Clavenna, A; Bonati, M; Ronfani, L
(2011) Oral ondansetron versus domperidone for symptomatic treatment of vomiting during acute
gastroenteritis in children: multicentre randomized controlled trial
IF=0 [Article]
53) Meazzini, MC; Brusati, R; Diner, P; Gianni, E; Lalatta, F; Magri, AS; Picard, A; Sesenna, E
(2011) The importance of a differential diagnosis between true hemifacial microsomia and pseudo-
hemifacial microsomia in the post-surgical long-term prognosis
MAXILLOFACIAL SURGERY 39(1):10-16 IF=1.252 [Article]
Long-term results after surgical treatment of the mandibular asymmetry in growing children with hemifacial microsomia (HFM), whether with osteotomies or distraction osteogenesis, have mostly shown a tendency towards the recurrence of the asymmetry. In contrast, in the literature we find sporadic case reports where the long-term post-surgical follow-up of patients diagnosed as HFM, are surprisingly stable. All these reports refer to patients who have substantially no soft tissue involvement, but only severe mandibular ramus and condyle deformities. The phenotypes of these cases are unexpectedly similar. The authors suggest, that it is possible that all of these cases might be isolated hemimandibular hypoplasias, misdiagnosed as HFM, which present a normal functional matrix and, therefore, tend to grow towards the original symmetry. Differential diagnosis between true HFM and this HFM-like isolated hemimandibular hypoplasia (pseudo-HFM) is of great importance given the very different prognosis and it is possible through the collaboration between not only surgeons and orthodontists, but also of geneticists and dysmorphologists. (C) 2010 European Association for Cranio-Maxillo-Facial Surgery. 54) Mergoni, G; Ergun, S; Vescovi, P; Mete, O; Tanyeri, H; Meleti, M (2011) Oral
postinflammatory pigmentation: An analysis of 7 cases
Y CIRUGIA BUCAL 16(1):0-0 IF=0 [Article]
55) Metra, M; Zaca, V; Parati, G; Agostoni, P; Bonadies, M; Ciccone, M; Cas, AD; Iacoviello,
M; Lagioia, R; Lombardi, C; Maio, R; Magri, D; Musca, G; Padeletti, M; Perticone, F; Pezzali, N;
Piepoli, M; Sciacqua, A; Zanolla, L; Nodari, S; Filardi, PP; Cas, LD (2011) Cardiovascular and
noncardiovascular comorbidities in patients with chronic heart failure
CARDIOVASCULAR MEDICINE 12(2):76-84 IF=0.712 [Review]
56) Mormile, R; De Michele, M; Squarcia, U; Quaini, F (2011) Hypertrophic cardiomyopathy
in neonates of diabetic mothers: Indirect evidence for a model of apoptotic reversibility by
Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  57) Murtas, C; Bruschi, M; Carnevali, ML; Petretto, A; Corradini, E; Prunotto, M; Candiano,
G; degl'Innocenti, ML; Ghiggeri, GM; Allegri, L (2011) In vivo characterization of renal auto-
antigens involved in human auto-immune diseases: The case of membranous glomerulonephritis
Renal auto-immune diseases represent a major source of morbidity in humans. For many years the knowledge on mechanisms of auto-immunity involving the kidney has been uniquely based on animal models. However, these findings often could not be readily translated to humans owing to notably difference in antigen expression by human podocytes. One example is Heymann nephritis (HN), the experimental model of human membranous glomerulonephritis (MGN), which is obtained in rats by injecting antibodies against megalin, a protein that is not present in human glomeruli. Human studies could not be done in the past since sequencing required too much material exceeding what obtainable from tissue biopsies in vivo. Research is now on the way to identify auto-antigens and isolate specific auto-antibodies in humans. New technology developments based on tissue microdissection and proteomical analysis have facilitated the recent discoveries, allowing direct analysis of human tissue in vivo. Major advances on the pathogenesis of MGN, the prototype for the formation and glomerular deposition of auto-antibodies, are now in progress. Two independent groups have, in fact, demonstrated the existence of specific IgG4 against phospholipase A2 receptor, aldose reductase and Mn-superoxide dismutase in glomerular eluates and in plasma of a prominent part of patients with MGN, suggesting a major role of these proteins as auto-antigens in human MGN. This review will focalize these aspects outlining the contribution of proteomics in most recent developments. 58) Napora, JK; Short, RG; Muller, DC; Carlson, OD; Odetunde, JO; Xu, XQ; Carducci, M;
Travison, TG; Maggio, M; Egan, JM; Basaria, S (2011) High-Dose Isoflavones Do Not Improve
Metabolic and Inflammatory Parameters in Androgen-Deprived Men With Prostate Cancer
JOURNAL OF ANDROLOGY 32(1):40-48 IF=2.344 [Article]
59) Nicolini, F; Fragnito, C; Molardi, A; Agostinelli, A; Campodonico, R; Spaggiari, I;
Beghi, C; Gherli, T
(2011) Heart surgery in patients on chronic dialysis: is there still room for
improvement in early and long-term outcome?
HEART AND VESSELS 26(1):46-54 IF=1.716
The purpose of this study was to review the outcome of dialysis-dependent patients undergoing cardiac surgery. We retrospectively reviewed 81 dialysis-dependent patients with a mean age of 62.5 +/- A 9.4 years who underwent cardiac operations. Mean EuroScore was 7.1 +/- A 3.9 (> 9 in 18 patients). Surgery included coronary artery bypass grafting (CABG) in 43 patients (53.1%), valve surgery in 16 (19.7%), combined CABG plus valve surgery in 19 (23.5%) and major aortic surgery in three patients. In-hospital mortality rate was 13.6%. Most of the deaths occurred in patients who underwent valve procedures or combined surgery. Preoperative New York Heart Association class IV, previous acute myocardial infarction, combined surgical procedures, major aortic surgery, age > 70 years, history of heart failure, female gender, the duration of dialysis a parts per thousand yen5 years and urgent/emergent surgery were associated with high relative risk for perioperative death. The actuarial survival was 72.2% at 5 years. Predictors of increased late mortality were heart failure, urgent/emergent surgery, the complexity of the surgical procedures (valve surgery, combined CABG + valve and major aortic surgery) and postoperative low cardiac output syndrome. In dialysis-dependent patients, CABG has an acceptable risk. Results in patients affected by valve lesions associated or not with coronary artery disease are improved by an early Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  referral to surgery, before the onset of symptoms of heart failure. 60) Ojeaburu, JV; Ito, T; Crafa, P; Bordi, C; Jensen, RT (2011) Mechanism of Acid
Hypersecretion Post Curative Gastrinoma Resection
56(1):139-154 IF=1.838 [Article]
61) Oretti, G; Giordano, D; Di Leila, F; Gradoni, P; Zendri, E; Ferri, T (2011) Unilesional
pemphigus vulgaris of the scalp after cochlear implantation
OTOLARYNGOLOGY 32(1):80-81 IF=1.016 [Article]
62) Patrelli, TS; Berretta, R; Gizzo, S; Pezzuto, A; Franchi, L; Lukanovic, A; Nardelli, GB;
Modena, AB (2011) CA 125 serum values in surgically treated endometriosis patients and its
relationships with anatomic sites of endometriosis and pregnancy rate
STERILITY 95(1):393-396 IF=3.97 [Editorial Material]
63) Piccini, JP; Schulte, PJ; Pieper, KS; Mehta, RH; White, HD; de Werf, FV; Ardissino, D;
Califf, RM; Granger, CB; Ohman, EM; Alexander, JH (2011) Antiarrhythmic drug therapy for
sustained ventricular arrhythmias complicating acute myocardial infarction
MEDICINE 39(1):78-83 IF=6.373 [Article]
64) Pinto, C; Ardizzoni, A; Betta, PG; Facciolo, F; Tassi, G; Tonoli, S; Zompatori, M;
Alessandrini, G; Magrini, SM; Tiseo, M; Mutri, V (2011) Expert Opinions of the First Italian
Consensus Conference on the Management of Malignant Pleural Mesothelioma
IF=2.206 [Review]
65) Previtali, M; Repetto, A; Camporotondo, R; Citro, R; Faggiano, P; Bovelli, D; Baldini, E;
Pasquetto, G; Ascione, L; Vignali, L; Rosso, R; Baralis, G; Rossi, ML; Ferlini, M; Bossone, E;
Panciroli, C; Della Rovere, F; Visconti, LO; Klersy, C (2011) Clinical Characteristics and
Outcome of Left Ventricular Ballooning Syndrome in a European Population
JOURNAL OF CARDIOLOGY 107(1):120-125 IF=3.575 [Article]
66) Ridolo, E; De Angelis, GL; Dall'Aglio, P (2011) EOSINOPHILIC ESOPHAGITIS AFTER
ASTHMA & IMMUNOLOGY 106(1):73-74 IF=2.457 [Letter]
Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  67) Rossi, S; Ravetta, V; Rosa, L; Ghittoni, G; Viera, FT; Garbagnati, F; Silini, EM; Dionigi, P;
Calliada, F; Quaretti, P; Tinelli, C (2011) Repeated Radiofrequency Ablation for Management of
Patients with Cirrhosis with Small Hepatocellular Carcinomas: A Long-Term Cohort Study
HEPATOLOGY 53(1):136-147 IF=10.84 [Article]
In most patients with cirrhosis, successful percutaneous ablation or surgical resection of hepatocellular carcinoma (HCC) is followed by recurrence. Radiofrequency ablation (RFA) has proven effective for treating HCC nodules, but its repeatability in managing recurrences and the impact of this approach on survival has not been evaluated. To this end, we retrospectively analyzed a prospective series of 706 patients with cirrhosis (Child-Pugh class <= B7) who underwent RFA for 859 HCC <= 35 mm in diameter (1-2 per patient). The results of RFA were classified as complete responses (CRs) or treatment failures. CRs were obtained in 849 nodules (98.8%) and 696 patients (98.5%). During follow-up (median, 29 months), 465 (66.8%) of the 696 patients with CRs experienced a first recurrence at an incidence rate of 41 per 100 person-years (local recurrence 6.2; nonlocal 35). Cumulative incidences of first recurrence at 3 and 5 years were 70.8% and 81.7%, respectively. RFA was repeated in 323 (69.4%) of the 465 patients with first recurrence, restoring disease-free status in 318 (98.4%) cases. Subsequently, RFA was repeated in 147 (65.9%) of the 223 patients who developed a second recurrence after CR of the first, restoring disease-free status in 145 (98.6%) cases. Overall, there were 877 episodes of recurrence (1-8 per patient); 577 (65.8%) of these underwent RFA that achieved CRs in 557 (96.5%) cases. No procedure-related deaths occurred in 1,921 RFA sessions. Estimated 3- and 5-year overall and disease-free (after repeated RFAs) survival rates were 67.0% and 40.1% and 68.0 and 38.0%, respectively. Conclusion: RFA is safe and effective for managing HCC in patients with cirrhosis, and its high repeatability makes it particularly valuable for controlling intrahepatic recurrences. 68) Rossini, A; Frati, C; Lagrasta, C; Graiani, G; Scopece, A; Cavalli, S; Musso, E; Baccarin, M;
Di Segni, M; Fagnoni, F; Germani, A; Quaini, E; Mayr, M; Xu, QB; Barbuti, A; DiFrancesco, D;
Pompilio, G; Quaini, F; Gaetano, C; Capogrossi, MC (2011) Human cardiac and bone marrow
stromal cells exhibit distinctive properties related to their origin
RESEARCH 89(3):650-660 IF=5.801 [Article]
69) Serra, W; Musiari, L; Ardissino, D; Gherli, T; Montanari, A (2011) Benefit of
prostaglandin infusion in severe heart failure Preliminary clinical experience of repetitive
Background: Prostaglandin E1 (PGE1) is a potent vasodilating drug, which has been used in treatment of primary pulmonary hypertension. However intravenous PGE1 infusion may be of benefit and also has been proposed as a therapeutic tool in patients with end-stage heart failure. The aim of this prospective not randomized study was to assess the clinical and instrumental effects of this agent in patients with severe heart failure and pulmonary hypertension. Methods: To investigate the effects of PGE1 in congestive heart failure we selected 22 consecutive patients (16 males, 6 females, mean age 63 +/- 2 years) in the mean NYHA class III, because they had pulmonary hypertension (PAPs>3 m/s and left ventricular ejection fraction (LVEF) <= 35% by echocardiography. A control group of 23 patients (19M, 4F mean age 62 +/- 5 years; 9 patients were in the NYHA class IV and 14 in the NYHA class III), with the same instrumental and clinical data, received an optimized oral treatment with beta-blockers, ACE-inhibitors, furosemide and digitalis. Right heart catheterization was performed to confirm and determine the type of pulmonary hypertension, before starting the PGE1 infusion. Clinical and echocardiography evaluation was performed Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  during follow-up. PGE1 was infused at a mean dose of 10 ng/kg/min for a total of 24 h over three consecutive days every three months. Results: Right heart catheterization confirmed a high systolic pulmonary pressure in all patients; pre-capillary pulmonary hypertension (mean PAP>25 mm/Hg) was 25%. During a mean follow-up of 36 +/- 6 months, 16 patients died (10 in the control group and 6 in the PGE1 group). The Kaplan-Meier 3-years survival analysis was not statistically significant (Log-rank test), but at 2 months survival rates began to diverge; 36 months survival: 72.7% in the PGE1 group and 56% in the control group. The mean LVEF increased from 25.78% to 32.1% in the PGE1 group and from 23.38% to 26.15 in the control group (p<0.001); the NYHA mean class improved from 3.18 to 2.24 in the PGE1 group and from 3.46 to 3.38 in the control group (p<0.05). The PAP decreased from 57.65 to 40.82 mm/Hg (p<0.001). An AICD was implanted in 3 patients in the first group and in 5 patients in the control group. Two patients were added to the heart transplantation list. Conclusion: These preliminary data suggest that intermittent PGE1 infusion in patients with advanced congestive heart failure and high pulmonary pressure is able to improve NYHA mean class (p<0.05), ventricular contractility (LVEF p<0.001), pulmonary pressure and clinical data. It hasn't been associated to morbid events or increased risk of death. 70) Street, ME; Viani, I; Ziveri, MA; Volta, C; Smerieri, A; Bernasconi, S (2011) Impairment
of insulin receptor signal transduction in placentas of intra-uterine growth-restricted newborns and
its relationship with fetal growth
52 IF=3.539 [Article]
Objective: Intra-uterine growth restriction (IUGR) is related to a higher incidence of type 2 diabetes mellitus. We previously reported reduced adiponectin and increased interleukin 6 (IL6) concentrations in IUGR placentas, which are features of insulin resistance. We aimed to investigate placental insulin receptor (IR) function and activation in human placenta and subsequently the relationships of insulin signalling peptides with placental protein content in IL6, insulin, resistin and adiponectin, and with parameters of fetal growth. Design and methods: Whole villous tissue was collected from 18 IUGR and 24 appropriate for gestational age (AGA) placentas of comparable gestational age. Insulin signalling peptides, suppressors of cytokine signalling-2 (SOCS2), insulin, adiponectin, resistin, and IL6 concentrations were determined by using western immunoblotting or specific research kits. Results: The amount of total IR was similar in both groups but activated IR significantly higher in IUGR. Total IR substrate-1 (IRS1) was increased in IUGR, whereas total IRS2 and activated IRS1 were similar. AKT content was reduced and activated AKT was undetectable in IUGR placentas. c-Jun N-terminal kinase content was reduced in IUGR. Total and activated ERK1/2 was similar in IUGR and AGA groups, and total SOCS2 was increased in IUGR. IL6 lysate concentrations correlated with AKT content and activated IR. Correlations were found also with adiponectin and resistin. SOCS2 correlated negatively with all growth parameters at birth. Conclusions: IR was more activated in placentas of IUGR compared with AGA; however, signal transduction downstream of the receptor was impaired. The increase in activated IR could be in favour of a compensatory mechanism to increase insulin sensitivity. Close relationships of insulin action in placenta with fetal growth were shown. 71) Suppiah, R; Flossmann, O; Mukhtyar, C; Alberici, F; Baslund, B; Brown, D; Hasan, N; Holle,
J; Hruskova, Z; Jayne, D; Judge, A; Little, MA; Merkel, PA; Palmisano, A; Seo, P; Stegeman, C;
Tesar, V; Vaglio, A; Westman, K; Luqmani, R (2011) Measurement of damage in systemic
vasculitis: a comparison of the Vasculitis Damage Index with the Combined Damage Assessment
ANNALS OF THE RHEUMATIC DISEASES 70(1):80-85 IF=8.111 [Article]
Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  72) Targher, G; Chonchol, M; Zoppini, G; Franchini, M (2011) Hemostatic Disorders in Type
1 Diabetes Mellitus
IF=3.214 [Article]
This review summarizes current knowledge of the adverse effects of type 1 diabetes mellitus on coagulation and fibrinolysis. Although further larger studies are needed to provide more definitive information, patients with type 1 diabetes exhibit a proinflammatory/procoagulant condition deriving from increased platelet adhesiveness, activation of the coagulation system, and decreased plasma fibrinolytic potential. This review also focuses on recent data from large prospective studies suggesting a strong association between procoagulant imbalance and development of chronic vascular complications in people with type 1 diabetes. It is likely that a greater appreciation of the intimate interactions between endothelial integrity, coagulation and fibrinolytic factors, and platelets in type 1 diabetes will provide a greater understanding of the risk of developing cardiovascular disease and microvascular complications such as nephropathy and retinopathy in this patient population. 73) Tiseo, M; Gelsomino, F; Boggiani, D; Bortesi, B; Bartolotti, M; Bozzetti, C; Sammarelli, G;
Thai, E; Ardizzoni, A (2011) EGFR and EML4-ALK gene mutations in NSCLC: A case report of
erlotinilb-resistant patient with both concomitant mutations
LUNG CANCER 71(2):241-243
IF=3.14 [Article]
The fusion gene EML4-ALK (echinoderm microtubule-associated protein-like 4 gene and the anaplastic lymphoma kinase gene) was recently identified as a novel genetic alteration in non-small cell lung cancer (NSCLC). EML4-ALK translocations correlate with specific clinical and pathological features, in particular lack of EGFR and K-ras mutations, and may be associated with resistance to EGFR tyrosine-kinase inhibitors (TKIs). Here, we report a case of a patient with a concomitant EGFR mutation and ALK translocation resistant to erlotinib. Considering this report, ALK status should be investigated in unexplained cases of EGFR-TKI-resistance of EGFR mutated NSCLCs. 74) Tofighi, R; Johansson, C; Goldoni, M; Ibrahim, WNW; Gogvadze, V; Mutti, A; Ceccatelli, S
(2011) Hippocampal Neurons Exposed to the Environmental Contaminants Methylmercury and
Polychlorinated Biphenyls Undergo Cell Death via Parallel Activation of Calpains and Lysosomal
NEUROTOXICITY RESEARCH 19(1):183-194 IF=2.439 [Article]
75) Trujillo-Santos, J; Casa, JM; Casado, I; Samperiz, AL; Quintavalla, R; Sahuquillo, JC;
Monreal, M (2011) Thirty-day mortality rate in women with cancer and venous
thromboembolism. Findings from the RIETE Registry
IF=2.406 [Article]
Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  76) Tzani, P; Chetta, A; Olivieri, D (2011) Patient assessment and prevention of pulmonary
side-effects in surgery
Purpose The review deals with the clinical and functional assessment that is requested before surgical operations and with the pulmonary effects of surgery. Moreover, an evidence-based approach to prevent postoperative pulmonary complications is provided. Recent findings Spirometry is recommended for patients with a history of tobacco use or dyspnoea who are considered for cardiac or upper abdominal surgery and for all patients who are candidated for lung resection. For patients undergoing lung resection, low complication rates have been associated with a preoperative forced expiratory volume at first second value of at least 2 litres or 80% of the predicted value and at least 1.5 litres or 60% of the predicted value in the case of pneumonectomy or lobectomy, respectively. At-risk patients are recommended to undergo split lung function studies to estimate the residual parenchyma function after surgery. In patients with borderline estimated values, a cardiopulmonary exercise test is recommended to stratify further risk. Preventive interventions of postoperative pulmonary complications, including pain control, respiratory chest physiotherapy and continuous positive airway pressure may effectively reduce the occurrence of pulmonary complications. Summary Pulmonary functional assessment is relevant in patients candidated for surgery. The prevention of the postoperative pulmonary complications is one of the most important goals of the treatment of patients undergoing surgery. 77) Vaglio, A; Pipitone, N; Salvarani, C (2011) Chronic periaortitis: a large-vessel vasculitis?
Purpose of review Chronic periaortitis is characterized by a fibro-inflammatory process spreading from the abdominal aorta and the iliac arteries. Originally, chronic periaortitis was considered a localized inflammatory response to severe aortic atherosclerosis. However, subsequent studies have shown that chronic periaortitis may also involve other arteries and present with features of auto-immune diseases. This article reviews the issue of large-vessel involvement in chronic periaortitis and its implications in the pathogenesis and nosography of the disease. Recent findings In many reports, chronic periaortitis has been shown to involve not only the aorto-iliac axis but also other vascular segments such as the thoracic aorta, the proximal epiaortic arteries, the coronary, renal, and mesenteric arteries. Thoracic aorta involvement may manifest as thoracic periaortitis with or without aneurysmal dilatation, or simply as thoracic aorta aneurysm. Thoracic periaortitis can also be a feature of the so-called IgG4-related systemic disease, with which chronic periaortitis may sometimes be associated. Histopathologic studies of chronic periaortitis show adventitial inflammation and fibrosis, vasculitis of vasa vasorum, and adventitial lymphoid follicles with germinal centers, suggesting that chronic periaortitis could be a primary aortitis. Genetic studies have demonstrated an association with HLA-DRB1*03, a marker of auto-immunity, and with the CCR5 Delta 32 polymorphism, which has been mapped to a Th2 response. Taken together, these findings support the notion of a primary inflammatory or immune-mediated disorder. Summary Chronic periaortitis is an inflammatory or immune-mediated disorder characterized histopathologically by adventitial inflammation and clinically by variable involvement of different arteries, mainly of large caliber. These findings raise the issue of whether chronic periaortitis should be considered a large-vessel vasculitis. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011  78) Vaienti, L; Gazzola, R; Palitta, G; Vaienti, E (2011) The Role of Inconstant Perforating
Arteries in Harvesting Radial Flaps
127(1):484-485 IF=2.743 [Letter]
79) Van Linthout, S; Spillmann, F; Graiani, G; Miteva, K; Peng, J; Van Craeyveld, E; Meloni, M;
Tolle, M; Escher, F; Subasiguller, A; Doehner, W; Quaini, F; De Geest, B; Schultheiss, HP;
Tschope, C (2011) Down-regulation of endothelial TLR4 signalling after apo A-I gene transfer
contributes to improved survival in an experimental model of lipopolysaccharide-induced
80) Vashist, P; Talwar, B; Gogoi, M; Maraini, G; Camparini, M; Ravindran, RD; Murthy, GV;
Fitzpatrick, KE; John, N; Chakravarthy, U; Ravilla, TD; Fletcher, AE (2011) Prevalence of
Cataract in an Older Population in India The India Study of Age-related Eye Disease
OPHTHALMOLOGY 118(2):272-0 IF=5.491 [Article]
81) Vescovi, M; Mengarelli, M; Franchini, M (2011) Self-perceived health status in blood
BLOOD TRANSFUSION 9(1):50-59 IF=0 [Article]
Background. Relationships between self-perceived health status and mortality, morbidity, disability and health care utilisation have been well established. The self-perceived health status of blood donors has, however, never been assessed. Materials and methods. Using the Short-Form 12 questionnaire, we assessed self-perceived health status in a sample of blood donors. Results. The mean values for the Physical Component Summary (PCS) score were similar for males and females, being 54 versus 54.12, respectively. There was, however, a gender difference for the Mental Component Summary (MCS) score, with women reporting a lower mean score. Young blood donors had the lowest mean score for the MCS, without gender differences. In all the other age categories, males had higher scores. Education did not seem to be as relevant as previously indicated in the literature. Our sample of blood donors included a certain number of overweight and obese subjects. The PCS scores were lowest in obese males and females. The highest MCS score was found in the group of overweight males. Conclusion. Further research in this selected population could be indicated as a new way to improve understanding of self-perceived health status and the determinants of the intention to become or to remain a blood donor. 82) Vescovi, PP; Favaloro, EJ; Lippi, G; Garofano, M; Montagnana, M; Manzato, F; Franchini,
(2011) The Spectrum of Coagulation Abnormalities in Thyroid Disorders SEMINARS IN
THROMBOSIS AND HEMOSTASIS 37(1):7-10 IF=3.214 [Article]
The hemostatic balance is a complex system where the delicate equilibrium is regulated by several factors including hormones. A variety of endocrine disorders have been reported to be associated with coagulation abnormalities, ranging from mild laboratory changes to clinically relevant thrombotic or bleeding manifestations. In this review, we summarize the current knowledge on the main abnormalities of the coagulation and fibrinolytic systems associated with thyroid dysfunctions. Overall, although mostly based on uncontrolled studies, data in the literature suggest that patients with hyperthyroidism or subclinical hypothyroidism have a hypercoagulative state, whereas patients with overt hypothyroidism have a bleeding tendency. Azienda Ospedaliero-Universitaria di Parma – Gennaio – Febbraio 2011 


Ivomec – Cydectin - Advocate Ivomec (Ivermectin) darf aufgrund seines Wirkstoffes bei folgenden Rassen wegen eines Gendefekts (MDR 1 Defekt) nicht eingesetzt werden: Collies, Australian Shepherds, Old English Sheepdogs sowie verwandte Hunderassen. Folgen wären schwere neurologische Symptome wie Zittern, Gangstörungen, Erbrechen bis zu Koma und Tod. Ivomec und Dectomax sind i.d.R. n

Long-Term Follow-up of Chronic Hepatitis B Virus Infection in Children of Different Ethnic Origins George Marx,1 Steven R. Martin,1 1 Division of Gastroenterology, Hepatology and Nutrition, Jean-Francois Chicoine,2 and Fernando Alvarez1 Sainte-Justine Hospital; 2 Department of Pediatrics, University The natural history of chronic hepatitis B in children is influenced by mode of trans

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