Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
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hebdap EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) A. Positions and Honors.
Res. Assoc., Dept. of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
Asst. Prof., Dept. of Dermatology, Univ. of Pgh., Sch of Med, Pittsburgh, PA
Assoc. Prof., Dept. of Dermatology, Univ. of Pgh., Sch of Med, Pittsburgh, PA
Assoc. Prof., Dept. of Cell Biology and Physiology, Univ. of Pgh., Sch of Med, Pittsburgh, PA
1995-Present Assoc. Prof., Dept. of Otolaryngology, Univ. of Pgh. Sch of Med, Pittsburgh, PA, 1995-Present Director, Wound Healing Research Program, Dept. of Pediatric Otolaryngology, Children’s Hosp. of Pgh 1996-Present Member, Graduate Training Faculty, Univ. of Pgh., Sch of Med, Pittsburgh, PA 1999-Present Chair, 1999-Present Animal Research and Care Committee (IACUC), Children’s Hospital of Pittsburgh, Pittsburgh, PA 1999-2001 Member, Board of Directors of the Wound Healing Society (elected by society membership) 2001-Present Member, Faculty of the McGowan Institute for Regenerative Medicine, Univ. of Pgh., Pittsburgh, PA 2003-Present Member, Cellular & Molecular Pathol Graduate Training Prog, Univ. of Pgh, Sch of Med, Pittsburgh, PA 2005-2010 Vice
President/President-Elect/President/Past President of the Wound Healing Society
Asst. Prof., Dept of Communication Science and Disorders, Univ. of Pgh., School of Health and
2006-Present Editor-in-Chief for the journal Wound Repair and Regeneration. 2008-Present Member, Executive Committee, McGowan Institute for Regenerative Medicine, Univ. of Pgh., Pittsburgh,
2008-Present Assoc. Prof., Dept. of Pathology, Univ. of Pgh., Sch of Med, Pittsburgh, PA
B. Recent peer-reviewed publications (in chronological order). Selected from over 60 published papers. Hebda PA, Yandrasitz J, Fukami MH. Subcellular fractionation of pig platelets. Biochem Biophys Acta
2. Hebda PA, Behrman EJ, Barber GA. The guanosine 5'-diphosphate D-mannose: guanosine 5'-diphosphate L-
galactose epimerase of Chlorella pyrenoidosa: Chemical synthesis of guanosine 5'-diphosphate L-galactose and further studies of the enzyme and the reaction it catalyzes. Arch Biochem Biophys 1979;194:496-502.
Hebda PA, Morris NP, Hollister DW. Human cartilage collagens: comparison of cartilage collagens
with human Type V collagen. J Biol Chem 1982;257:7852-7856.
4. Hebda PA, Alstadt SP, Hileman WT, Eaglstein WH. Support and stimulation of epidermal cell outgrowth from porcine
skin explants by platelet factors. Brit J Dermatol 1986;115:529-541.
Hebda PA, Zitelli JA, Abell E. Epidermal cell outgrowth from CO2-laser and scalpel cut explants:
Implications for wound healing. J Dermatol Surg Oncol 1984;10:863-868.
6. Kaufman T, Nathan P, Levin M, Hebda PA, Eichenlaub EH, Korol B. Drug-loaded synthetic dressings: Effect on
contraction, epithelialization and collagen synthesis of deep second-degree experimental burns. Ann Plast Surg; 1985;14:420-427.
Hebda PA, Eaglstein WH. Effect of platelet homogenate on glycosaminoglycans production by
scleroderma and normal control fibroblasts in vitro. Brit J Dermatol 1985;113:237-243.
Hebda PA, Chung AE, Eaglstein WH. The effect of basement membrane entactin on epidermal cell
attachment and growth. J Invest Dermatol 1986;88;55-59.
9. Whiteside TL, Ferrarini M, Hebda PA, Buckingham RB. Heterogeneous synthetic phenotype of cloned scleroderma
fibroblasts may be due to a loss of regulation in the synthesis of connective tissues. Arthritis Rheum 1988; 31:1221-9.
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10. Hebda PA. Stimulatory effects of transforming growth factor-beta and epidermal growth factor on epidermal cell
outgrowth from porcine skin explant cultures. J Invest Dermatol 1988;91:440-445.
11. Hambley R, Hebda PA, Abell E, Cohen BA, Jegasothy BV. Wound healing of skin incisions produced by
ultrasonically vibrating knife, scalpel, electrosurgery and CO2 laser. J Dermatol SurgOncol 1988; 14:1213-1217.
12. Hung VC, Lee JY-Y, Zitelli JA, Hebda PA. The effects of topical tretinoin on epithelial wound healing. Arch Dermatol
13. Hebda PA, Klingbeil CK, Abraham JA, Fiddes JC. Basic fibroblast growth factor stimulation of epidermal wound
healing in pigs. J Invest Dermatol 1990;95:626-631.
14. Kane CJM, Hebda PA, Mansbridge JN, Hanawalt PC. Direct evidence for spatial and temporal regulation of
transforming growth factor ß, expression during cutaneous wound healing. J Cell Physiol 1991;148:157-173.
15. Hebda PA, Lee CI. Occlusive dressings for surgical and other acute wounds. Wounds 1992;4:84-87. 16. Hebda PA, Collins MA, Tharp MD. Mast cell and myofibroblast in wound healing. Dermatol Clinics 1983;11:685-696. 17. Yasumura S, Lin W-C, Weidmann E, Hebda P, Whiteside TL. Expression of interleukin-2 receptors on human
carcinoma cell lines and tumor growth inhibition by interleukin-2. Int J Cancer 1994; 59:225-234.
18. Skrabut EM, Hebda PA, Samuels JA, Richards SM, Edmunds T, Cunneen MF, Vaccaro CA, McPherson JM.
Removal of necrotic tissue with an ananain-based enzyme debriding preparation. Wound Rep Reg 1996;4:433-443.
19. Cao M, Westerhausen-Larson A, Niyibizi C, Kavalkovich K, Gergescun HI, Rizzo CF, Hebda PA, Stefanovic-Racic M,
Evans CH. Nitric oxide inhibits the synthesis of type II collagen without altering Co12A1 mRNA abundance: prolyl hydroxylase as a possible target. Biochem J 1997;324:305-310.
20. Hebda PA, Alper CM, Doyle WJ, Burckart GJ, Diven WF, Zeevi A. Upregulation of mRNA for inflammatory mediators
in middle ear mucosa delineated in a rat model of infectious otitis media. Ann Otol Rhinol Laryngol 1998;107:501-7.
21. Dohar JE, Klein EC, Betsch JL, Hebda PA. Fetal airway wound repair - a new frontier. Arch Otolaryngol Head Neck
22. Goldstein NA, Hebda PA, Klein EC, Dohar JE. Wound management of the airway mucosa: comparison with skin in a
rabbit model. Int J Pediatr Otorhinolaryngol 1998;45:223-235.
23. Dohar JE, Klein EC, Betsch JL, Hebda PA. Acquired subglottic stenosis-depth and not extent of the insult is the key. Int J Pediatr Otorhinolaryngol 1998;46:159-170.
24. Hebda PA, Flynn KJ, Dohar JE. An evaluation of the efficacy of enzymatic debriding agents for removal of necrotic
tissue and promotion of healing in porcine skin wounds. Wounds 1998;10:1-12.
25. Hebda PA, Dohar JE. Transplanted fetal fibroblasts--potential applications for cell therapy and tissue engineering. Otolaryngol Head Neck Surg 1999;121:245-51.
26. Darden DL, Hu FZ, Ehrlich MD, Gorry MC, Dressman D, Li H-S, Whitcomb DC, Hebda PA, Dohar JE, Ehrlich GD.
RNA differential display of scarless wound healing in fetal rabbit indicates downregulation of a CCT chaperonin subunit and upregulation of a glycophorin-like gene transcript. J Pediatr Surg 2000;35:406-419.
27. Alper CM, Dohar JE, Gulhan M, Ozunlu A, Sjobak D, Hebda PA, Swarts JD. Treatment of chronic suppurative otitis
media with topical tobramycin and dexamethasone. Arch Otolaryngol Head Neck Surg 2000;126:165-173.
28. Li HS, Hebda PA, Kelly LA, Ehrlich GD, Whitcomb DC, Dohar JE. Prostaglandin EP4 receptor messenger RNA is
overexpressed in fetal rabbit skin wounds. Otolaryngol Head Neck Surg 2000; 126:1337-1343.
29. Radfar AJ, Rosas FR, Robbins PD, Huard J, Dohar JE, Hebda PA. Viral transduction of cells and transplantation into
the dermis of immunocompetent and immunodeficient (SCID) mice to determine gene expression profile and differential donor cell survival. Wound Repair Regen 2000;8:503-510.
30. Hebda PA, Lo C-Y. The effects of active ingredients of standard debriding agents—papain and collagenase—on
digestion of native and denatured collagenous substrates, fibrin and elastin. Wounds 2001;13:190-194.
31. Hebda PA, Burckart GJ, Swarts JD, Zeevi A, Moody SA, Zheng HX, Doyle WJ, Alper C. Inflammation in
otitis media:effect of ten pharmacologic probes in a rat model. J Pediatr Pharmacol Ther 2002;7:54-63.
32. Hebda PA, Burckart GJ, Alper C, Swarts JD, Antonio S, Zeevi A, Doyle WJ. Effects of ten pharmacologic probes on
mRNA levels of iNOS and selected inflammatory cytokines in a rat model of acute otitis media. Acta Oto-Laryngologica 2002; 122:255-61.
33. Piltcher OB, Swarts JD, Magnuson K, Alper CM, Doyle WJ, Hebda PA. A rat model of otitis media with effusion
caused by eustachian tube obstruction with and without S. pneumoniae infection: Methods and disease course. Otolaryngol Head Neck Surg 2002;126:490-98.
34. Hebda PA, Piltcher OB, Swarts JD, Alper CM, Zeevi A, Doyle WJ. Cytokine profiles in a rat model of otitis media with
effusion caused by eustachian tube obstruction with and without S. pneumoniae infection. Laryngoscope 2002;112:1657-1662.
35. Li HS, Doyle WJ, Swarts JD, Hebda PA. Suppression of epithelial ion transport transcripts during pneumococcal
acute otitis media in the rat. Acta Otolaryngol 2002;122:488-94.
36. Sandulache VC, Hebda PA, Zhou Z, Sherman A, Dohar JE. Cell Therapy: Impact of transplanted fibroblasts on
rabbit skin wounds. ArchOtolaryngol Head Neck Surg 2003; 129:345-350.
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37. Mandell DL, Devor DC, Madia JV, Lo C-Y, Hake H, Hebda PA. The effect of changes in ambient oxygen
concentration on the bioelectric properties of middle ear mucosa. Am J Physiol 2003;285:C618-C622.
38. Hebda PA, Whaley D, Kim HG, Wells A. Absence of inhibition of cutaneous wound healing in mice by oral
doxycycline. Wound Rep Reg 2003;11:373-379.
39. Li HS, Doyle WJ, Swarts JD, Lo CY, Hebda PA. Mucosal expression of genes encoding possible upstream
regulators of Na+ transport during pneumococcal otitis media. Acta Otolaryngol 2003;123:575-582.
40. Verdolini K, Rosen CA, Branski RC, Hebda PA. Shift in biochemical markers associated with wound healing in
laryngeal secretions following phonotrauma. Ann Otol Rhinol Laryngol 2003; 112:1021-1025.
41. Satish L, Babu M, Tran KT, Hebda PA, Wells A. Keloid responsiveness to epidermal growth factor and activation of
downstream intracellular signaling pathways. Wound Rep Reg 2004;12:183-92.
42. Branski RC, Rosen CA, Verdolini K, Hebda PA. Markers of wound healing in vocal fold secretions from patients with
laryngeal pathology. Ann Otol Rhino Laryngol; 2004;113:23-29.
43. Li-Korotky HS, Swarts JD, Hebda PA, Doyle WJ. Cathepsin gene expression profile in rat acute pneumococcal otitis
media. Laryngoscope; 2004;114:1032-1036.
44. Branski RC, Rosen CA, Verdolini K, Hebda PA. Biochemical markers associated with acute vocal fold wound
healing: A rabbit model. J Voice; 2005;19:283-9.
45. Branski RC, Rosen CA, Verdolini K, Hebda PA. Acute vocal fold wound healing in a rabbit model. Ann Otol Rhinol
46. Branski RC, Sandulache VC, Dohar JE, Hebda PA. Mucosal wound healing in a rabbit model of subglottic stenosis:
biochemical analysis of secretions. Arch Otolaryngol Head Neck Surg;2005;131:153-7.
47. Sandulache VC, Dohar JE, Hebda PA. Adult-fetal fibroblast interactions: effects on cell migration and implications for
cell transplantation. Cell Transplantation 2005;14:331-7.
48. Chen A, Li H-S, Hebda PA, Zeevi A, Swarts JD. Gene expression profiles of early pneumococcal otitis media in the
rat. Int J Ped Otorhinolaryngol 2005;69:1383-1393.
49. Dohar JE, Hebda PA, Veeh R, Awad M, Costerton JW, Hayes J, Ehrlich GD. Mucosal biofilm formation on middle-
ear mucosa in a non-human primate model of chronic suppurative otitis media. Laryngoscope 2005;115:1469-72.
50. Sandulache VC, Dohar JE, Hebda PA. Fibroblast transplantation in the airway: implications for subglottic stenosis. Arch Otolaryngol Head Neck Surg. 2005;131:1090-1096.
51. Li-Korotky HS, Hebda PA, Kelly LA, Lo CY, Dohar JE. Identification of a pre-mRNA splicing factor arginine/serine-
rich 3 (Sfrs3) and co-expression with fibronectin in fetal and postnatal rabbit airway mucosal and skin wounds. Biochim Biophys Acta 2005;1762(1):34-45.
52. Branski RC, Verdolini K, Sandulache V, Rosen CA, Hebda PA. Vocal fold wound healing: A review for clinicians. J
53. Satish L, James Lyons-Weiler J, Hebda PA, Wells A. Gene expression patterns in isolated keloid fibroblasts. Wound
54. Sandulache VC, Parekh A, Li-Korotky HS, Dohar JE, Hebda PA. Prostaglandin E2 differentially modulates human
fetal and adult dermal fibroblast migration and contraction: implication for wound healing. Wound Rep Reg 2006;14:633-643.
55. Krishna P, Rosen CA, Branski RC, Wells AH, Hebda PA. Primed fibroblasts and exogenous decorin: Potential
treatments for subacute vocal fold scar. Otolaryngol Head Neck Surg 2006;135:937-945.
56. Sandulache VC, Parekh A, Li-Korotky HS, Dohar JE, Hebda PA. Prostaglandin E2 inhibits keloid fibroblast migration,
contraction and TGFβ1 induced collagen synthesis, but endogenous prostaglandin E2 synthesis is impaired. Wound Rep Reg 2007;15:122-133.
57. Branski, RC, Perera P, Verdolini K, Rosen CA, Hebda PA, Agarwal S. Dynamic biomechanical strain inhibits IL-1β-
induced inflammation in vocal fold fibroblasts. J Voice 2007;21:651-660.
58. Chafin JB, Sandulache VC, Dunkelbarger JL, Otteson TD, Hoffmann P, Hebda PA, Dohar JE. Graded CO2 laser
induced subglottic injury in the rabbit model. Arch Otolaryngol Head Neck Surg 2007;133:358-364.
59. Sandulache VC, Chafin JB, Li-Korotky HS, Otteson T, Dohar JE, Hebda PA. Elucidating the role of interleukin-1β
and prostaglandin E2 in upper airway mucosal wound healing. Arch Otolaryngol Head Neck Surg 2007;133:365-374.
60. Hebda PA, Yuksel S, Dohar JE. The effects of ciprofloxacin-dexamethasone on myringotomy wound healing.
61. Li-Korotky HS, Kelly LA, Piltcher O, Hebda PA, Doyle WJ. Evaluation of microbial RNA extractions from
Streptococcus pneumoniae. J Microbiol Methods 2007;68:342-348.
62. Parekh A, Sandulache VC, Lieb AS, Dohar JE, Hebda PA. Differential regulation of free floating collagen gel
contraction by human fetal and adult dermal fibroblasts in response to prostaglandin E2 mediated by an EP2/cAMP-dependent mechanism. Wound Rep Reg 2007;15:390-398.
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63. Yates CC, Whaley D, Kulasekeran P, Hancock WW, Lu B, Bodnar R, Newsome J, Hebda PA, Wells A. Delayed
and deficient dermal maturation in mice lacking the CXCR3 ELR-negative CXC chemokine receptor. Am J Pathol 2007;171:484-495.
64. Li-Korotky HS, Hebda PA, Lo CY, Dohar JE. Age-dependent expression of fibronectin variants in airway and skin
wounds. Arch Otolaryngol Head Neck Surg 2007;133:919-924.
65. Sandulache VC, Parekh A, Dohar JE, Hebda PA. Fetal dermal fibroblasts retain a hyperactive migratory and
contractile phenotype under 2-and 3-dimensional constraints compared to normal adult fibroblasts. Tissue Eng 2007;13:2791-2801.
66. Otteson TD, Sandulache VC, Barsic M, DiSilvio GM, Hebda PA, Dohar JE. Acute and chronic changes in the
subglottis induced by graded CO2 laser injury in the rabbit airway. Arch Otolaryngol Head Neck Surg 2008;134:1-9.
67. Li NYK, Verdolini K, Clermont G, Mi Q, Hebda PA, Rubinstein EN, Vodovotz Y. A patient-specific in silico model of
inflammation and healing tested in acute vocal fold injury. PLoS ONE 3(7):e2789.
68. Yates CC, Whaley D, Yen A, Kulesekaran P, Hebda PA, Wells A. (2008) ELR-negative CXC chemokine CXCL11
(IP-9/I-TAC) facilitates dermal and epidermal maturation during wound repair. Am J Pathol 173:643-652.
69. Yates CC, Whaley D, Hooda S, Hebda PA, Bodnar RJ, Wells A. (2008) Delayed re-epithelialization and basement
membrane regeneration after wounding in mice lacking CXCR3. Wound Rep Reg (in press).
70. Parekh A, Sandulache VC, Singh T, Cetin S, Sacks MS, Dohar JE, Hebda PA. (2008) Prostaglandin E2
differentially regulates contraction and structural reorganization of anchored collagen gels by human adult and fetal dermal fibroblasts. Wound Rep Reg (in press).
C. Research Support. Ongoing Research Support RESEARCH GRANTS: Subglottic Stenosis and Laryngotracheal Mucosal Healing Principal Investigator: Patricia A. Hebda, Ph.D. Agency: NIH/NIDCD Type: R01 DC007437 Period: 7/01/06 – 6/30/10
The overall goal of this study is to determine the key responses to wound signals that lead to regenerative healing by fetal fibroblasts, rather than reparative or fibrotic healing by postnatal fibroblasts. Pathogenesis of Otitis Media with Effusion Principal Investigator: Patricia A. Hebda, Ph.D. Agency: NIH/NIDCD Type: R01 DC007197 Period: 4/1/06 – 3/31/11
This study proposes to test the hypothesis that transduction of the signal associated with middle ear underpressure initiates and sustains an inflammatory process that contributes to persistence of otitis media with effusion and to adverse changes in middle ear physiology. Dermal-epidermal Communication during Wound Healing Principal Investigator: A. Wells, M.D.,D.Sc. Principal Investigator of CHP Subcontract: Patricia A. Hebda, Ph.D. Agency: NIH/NIGMS Type: R01 GM63569 Period: 7/1/03 – 6/31/13
This project examines the interaction of dermal and epidermal components in the coordination of the cutaneous wound healing response. A Hybrid Model of Vocal Fold Inflammation Principal Investigator: K. Verdolini, Ph.D. Principal Investigator of CHP Subcontract: Patricia A. Hebda, Ph.D. Agency: NIH/NIDCD Type: R01 DC008290 Period: 4/01/07 – 3/30/12
The long-range goal is to generate a technology incorporating mathematical modeling that allows clinicians to prescribe ideal, patient-specific vocal exercise (or rest) programs to optimize tissue healing in both acute and chronic phonotrauma.
Armed Forces Institute of Regenerative Medicine (AFIRM)Wake Forest/University of Pittsburgh Consortium Program Director: A. Atala, Ph.D. (Wake-Forest Univ) Co-Director: A. Russell, Ph.D. (Univ Pittsburgh) Project 4.4: Regulation of Inflammation, Fibroblast Recruitment and Activity for Regenerative Healing Project Leader: Patricia A. Hebda, Ph.D. Agency: U.S. Army Medical Research and Materiel Command (USAMRMC), the Office of Naval Research (ONR) and the National Institute of Health (NIH) Type: R&D Contract Period: 03/10/08-03/09/13 Continuation Format Page
This is a 5-year multi-institution initiative, with the option for a 5-year renewal, to establish the Armed Forces Institute of Regenerative Medicine (AFIRM), integrating basic science, translational and clinical research and dedicated to repairing battlefield injuries through the use of regenerative medicine. TRAINING GRANTS: Research Training in Pediatric Otolaryngology Program Director: Margaretha L. Casselbrant, M.D., Ph.D. Preceptor: Patricia A. Hebda, Ph.D. Agency: NIH/NIDCD Type: 5T32 DC00021 Period: 7/1/00 – 6/30/10
This program seeks to train pediatric otolaryngologists to succeed in research and teaching in an academic otolaryngology, head and neck surgery department. Dr. Hebda serves as a mentor for laboratory research projects. Engineering Structured Tissue for Clinical Medicine Program Director: Robert T. Rubin, M.D. Mentor: Patricia A. Hebda, Ph.D. Agency: NIH/NIBIB Type: 1 T32 EB00424-01 Period: 9/30/02 – 6/30/08
This Institutional NRSA supports postdoctoral fellowship training in tissue engineering (TE) for clinical applications in medicine and surgery. Cellular Approaches to Tissue Engineering and Regeneration (CATER) Program Director: Alan J. Russell, Ph.D. Mentor: Patricia A. Hebda, Ph.D. Agency: NIH/NIBIB Type: 1 T32 EB001026-01 Period: 7/01/03 – 6/30/08
This Institutional NRSA supports pre-doctoral training in cellular approaches for tissue engineering and regeneration for graduate students. Completed Research Support Prostaglandin E2: A Potential Therapy for Subglottic Stenosis Principal Investigator: Patricia A. Hebda, Ph.D. Agency: American Society of Pediatric Otolaryngology Type: seed grant Period: 7/1/04 – 6/30/05 This study uses an animal model for subglottic stenosis to investigate a novel approach to reducing mucosal fibrosis. This pilot study may provide a basis for new, non-cytotoxic, non-surgical therapies for subglottic stenosis. Biophysiological Mechanisms of Heightened Speech Principal Investigator: K. Verdolini, Ph.D. Principal Investigator of CHP Subcontract: Patricia A. Hebda, Ph.D. Agency: NIH/NIDCD Type: R01 DC005643 Period: 4/1/03 – 3/31/08
The long-term objective of this application is to study the different ways that “loud” or “heightened” speech can produce phonotrauma, or inflammation of the vocal folds, and to study the applications of voice training in reducing inflammation.
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INTRODUCCIÓN*Imperio y canon en William Henry HudsonLeila Gómez University of Colorado, Boulder En 1941, se publica en Argentina la pionera Antología de Guillermo Enrique Hudson con estudios críticos sobre su vida y su obra , con textos de Fernando Pozzo, E. Martínez Estrada, Jorge Casares, Jorge Luis Borges, H. J. Massingham, V. S. Pritchett y Hugo Manning. Fue este uno de los principales
SAFETY DATA SHEET Conforms to Regulation (EC) No. 1907/2006 (REACH), Annex II - United Kingdom (UK) METALLIC ZINC 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Product name Synonyms : Zinc Metal Sheets; Zinc Metal Shot; Zinc Metal Strips REACH Registration Chemical Formula 1.2 Relevant identified uses o