A Parent’s Guide to Sports-Related Skin Infections Prepared by: Nick Esmonde, MS-3 OHSU School of Medicine Pioneer Memorial Hospital Preceptor: Russell Nichols, MD This guide was designed to inform parents of student-athletes about the most common or serious sports-related skin infections. If an athlete has new skin lesions or infections, they should see their primary care doctor for evaluation
Bardzo tanie apteki z dostawą w całej Polsce kupic cialis i ogromny wybór pigułek.
6488_14_p97-102THE JOURNAL OF ALTERNATIVE AND COMPLEMENTARY MEDICINE
Volume 13, Number 1, 2007, pp. 97–102
Mary Ann Liebert, Inc.
The Mushroom Agaricus blazei Murill in Combination with Metformin and Gliclazide Improves Insulin Resistance in Type 2 Diabetes: A Randomized, Double-Blinded, CHUNG-HUA HSU, M.D., Ph.D.,1–4 YANG-LI LIAO, M.D.,2 SU-CHING LIN, M.D.,2 KUNG-CHANG HWANG, M.D.,3 and PESUS CHOU, Dr.P.H.4 Background: Complementary and alternative medicine use in adults with type 2 diabetes is popular. Al-
though most of the herbs and supplements appear to be safe, there is still insufficient evidence that demon-strates their definitive beneficial effects. This study was done to determine whether the supplement of Agari-cus blazei Murill extract improves insulin resistance in type 2 diabetes.
Materials and Methods: This study was a clinical randomized, double-blind, placebo-controlled trial. Of a
population of 536 registered diabetes patients with 72 subjects (1) aged between 20 and 75 years, (2) beingChinese, (3) having type 2 diabetes for more than 1 year, and (4) having been taking gliclazide and metforminfor more than 6 months were enrolled in this study. The enrolled patients were randomly assigned to either re-ceiving supplement of Agaricus blazei Murill (ABM) extract or placebo (cellulose) 1500 mg daily for 12 weeks.
Homeostasis model assessment for insulin resistance (HOMA-IR) was used as the major outcome measure-ment.
Results: At the end of the study, subjects who received supplement of ABM extract (n ϭ 29) showed sig-
nificantly lower HOMA-IR index (3.6[standard deviation, 2.5] versus 6.6[standard deviation, 7.4], p ϭ 0.04)than the control group (n ϭ 31). The plasma adiponectin concentration increased 20.0(standard deviation,40.7)% in the ABM group after 12 weeks of treatment, but decreased 12.0(20.0)% among those taking theplacebo (p Ͻ 0.001).
Conclusions: Supplement of ABM extract improves insulin resistance among subjects with type 2 diabetes.
The increase in adiponectin concentration after taking AMB extract for 12 weeks might be the mechanism thatbrings the beneficial effect. Studies with longer periods of follow-up should be conducted in the future.
able to find a natural herbal medicine that can help boostinsulin resistance but has less undesirable side effects.
Type 2 diabetes represents a heterogeneous group of dis- The mushroom Agaricus blazei Murill (ABM) is a nat-
orders characterized by increased insulin resistance.1 ural food, which has been used as a health care product for Lifestyle, diet, obesity, and family history of diabetes have the prevention of a range of illness including cancer, dia- been associated with the development of insulin resistance, betes, arteriosclerosis, and chronic hepatitis. It has been re- although the molecular pathway remains unknown.2 Thia- ported that ABM has beneficial effects in fighting can- zolidinediones (TZD) have been found to improve periph- cer,6–10 virus,11 and Streptococcus pneumonia infection in eral insulin resistance and has been employed for treating mice12 as well as enhancing antibody production of vac- type 2 diabetes.3,4 However, some adverse side effects on cine.13,14 Rich polysaccharides such as ␤-glucans are found liver function have also been reported.5 Hence, it is desir- to be the main compounds of ABM.14–17 ␤-Glucans from 1Department of Chinese Medicine, Taipei Hospital, Taiwan.
2Department of Endocrinology, Taipei Hospital, Taiwan.
3Department of Pediatrics, Taipei Hospital, Taiwan.
4Community Medicine Research Center and Institute of Public Health, National Yang-Ming University, Taipei, Taiwan.
HSU ET AL.
ABM have demonstrated antidiabetic activity.17 An animal dom number between 0.50 and 0.99 would be assigned to model study and a pilot study have both indicated its bene- the placebo group with cellulose given. The same opaque ficial effect in type 2 diabetes. Hence, we conducted this capsules containing either dried powdered ABM extract or clinical trial to examine whether ABM extract given as a placebo (cellulose) were administered to the subjects by a supplement can improve insulin resistance in type 2 dia- research assistant blinded to the contents in the capsules. All patients were treated in the same fashion.
RESEARCH DESIGN AND METHODS
Homeostasis model assessment for insulin resistance (HOMA-IR) [fasting glucose (mmol/L) ϫ fasting insulin (UI/L)/22.5] was used as the major outcome measure- The trial was conducted from July 1, 2005 through De- ment.18,19 At baseline and after 12 weeks of treatment, an- cember 31, 2005 in the Taipei Hospital, Taiwan. Of a pop- thropometric measurements, blood pressure, fasting glucose, ulation of 536 registered patients with diabetes, 72 subjects hemoglobulin A1C% (HbA1C), insulin, adiponectin and met with the inclusion criteria: (1) aged between 20 and 75 plasma lipoproteins (triglyceride, cholesterol, choles- years; (2) being Chinese; (3) having type 2 diabetes for more terol–high-density lipoprotein, and cholesterol–low-density than 1 year; and (4) having been taking gliclazide and met- lipoprotein) of both groups were measured. The change (%) formin for more than 6 months were enrolled in this study; in concentration of adiponectin after the 12-week treatment and exclusion criteria were as follows: (1) aminotransferases aspartate or aminotransferases alanine Ͼ80 IU/L, serum cre- All measurements were made at 8 AM to 9 AM after an atinine Ͼ2.0 mg/dL; (2) prolacting or pregnant women, heart overnight fast using standardized methods. A sample of failure, acute myocardic infarct, stroke, and heavy injuries; whole blood was drawn and centrifuged at 4°C, and a 1-mL and (3) any other conditions not suitable for trial as evalu- aliquot of serum was rapidly frozen (Ϫ80°C) for subsequent ated by the physician. The protocol was approved by the hormone analysis. The plasma adiponectin concentration Human Ethics Committee of our hospital. Informed consent was measured by a radioimmunoassay kit (Linco Research, was obtained from all the enrolled patients. The patients Inc., St. Charles, Missouri). This kit employs the double-an- were instructed to maintain an isocaloric diet and their pre- tibody/polyethylene glycol technique using 125I-lableled vious eating habits during the study period. All subjects were adiponectin and a multispecies adiponectin rabbit antis- free to withdraw at any time during the course of the study.
erium. Plasma insulin levels were measured using a com-mercially available radioimmunoassay (Linco ResearchInc.). The intra- and interassay coefficients of variation were Preparation of sample and treatment 3.1% and 4.9%, respectively. The limit of sensitivity is 0.5 ABM is a health care product popularly used in Taiwan.
Our ABM extract samples, obtained from Eng Chiao Bio-Technology Co. Ltd., Taiwan, were extracted from dried fungal bodies of ABM according to the preset standard pro-cedures with certificate of analysis given. The placebo given The data were analyzed with SPSS software (version to the control group comprised pure microcrystalline cellu- 11.5, SPSS Software, Inc., Chicago, IL). Paired t tests were lose. The subjects were asked to take one capsule contain- used to examine differences within-group at 0 to 12 weeks.
ing 500 mg of either ABM extract or cellulose three times Student t test was used to examine the main outcome, de- each day for 12 weeks. The capsule was taken with gli- mographic data, and other measurements between group clazide 30 minutes before eating and metformin was taken means. Chi-square test was employed for gender compari- 30 minutes after eating. During the study period, the sub- son between groups. All p values were two-tailed, and the jects should keep taking the same dose of gliclazide and met- ␣ level of significance was set at 0.05. We estimated in formin except when hypoglycemia occurs, in which case the power 0.8 that each group required 28 subjects.
dose of gliclazide or metformin should be reduced immedi-ately.
All subjects were randomly assigned to one of the two groups. A random number between 0.0 and 0.99 would be Seven subjects of the ABM extract group and five sub- generated by the computer for each patient. Patients with a jects of the placebo group withdrew because of personal rea- random number between 0.0 and 0.49 were assigned to the sons. In the end, 60 patients completed the study. Table 1 group with ABM extract given, whereas those with a ran- shows the demographic data, fasting serum glucose meta- AGARICUS BLAZEI SUPPLEMENT FOR INSULIN RESISTANCE
TABLE 1. CHARACTERISTICS OF SUBJECTS AT BASELINE HbA1c, hemoglobin A1c; HOMA-IR, homeostasis model assessment for insulin resistance; HDL, high-density lipoprotein; LDL, bolic factors, plasma lipoprotein, and clinical profiles of the groups at the time of entry. As can be seen, there were no Three subjects of the ABM extract group had hypo- significant differences in all the baseline measurements be- glycemia-like symptoms; 2 patients had the dose of oral hy- tween the ABM extract group and the placebo group.
poglycemia agent (OHA) reduced, whereas 1 patient with-drew. One subject of the placebo group also developed Within-group comparison at 12 weeks hypoglycemia-like symptoms. The dose of OHA was re- Table 2 shows the within-group comparisons at baseline duced and he stayed on in the study.
and after the 12-week treatment. According to the analysis Two subjects of the ABM extract group developed skin of the fasting serum glucose metabolic factors, the ABM ex- itching, 1 subject of the placebo group had skin allergy with tract group showed a significant difference in reduced papules, and another had nausea and fullness sensation. All HbA1C, insulin concentration, and homeostasis model as- 4 of them withdrew. No major adverse effects were noticed.
sessment for insulin resistance (HOMA-IR) index as well asincreased concentration of adiponectin, compared with theinitial values, whereas the placebo group showed significantdifference only in reduced adiponectin level compared with DISCUSSION
This study shows the benefits of ABM extract supple- ment for reducing the HOMA-IR index in subjects with type Between-group comparison at 12 weeks 2 diabetes treated with gliclazide and metformin.
At the end of 12 weeks (Table 2), there were no signifi- Insulin resistance, a common cause of type 2 diabetes, cant differences between the two groups in all the mea- implies impairment of insulin signaling in target tissues. It surements except insulin concentration and HOMA-IR in- has been reported that some OHA might influence insulin dex (p ϭ 0.05 and 0.04). Figure 1 shows a significant resistance.4,5,17,20–22 To avoid existing confounders and bias, difference between the ABM extract group and the placebo we examined a homogenous Chinese cohort who had had group (p Ͻ 0.001) in terms of change (%) in plasma type 2 diabetes for more than 1 year and had been taking adiponectin concentrations after 12 weeks of treatment.
gliclazide and metformin for more than 6 months.
HSU ET AL.
TABLE 2. CHARACTERISTICS OF SUBJECTS AFTER 12-WEEK TREATMENT Data are means (SD).
*p Ͻ 0.05 from baseline to the end (12 weeks) with paired t tests.
HbA1c, hemoglobin A1c; HOMA-IR, homeostasis model assessment for insulin resistance; HDL, high-density lipoprotein; LDL, Many animal model studies have demonstrated the anti- diabetic activity of ABM.17 After an animal model study in2003 and a pilot study had showed its beneficial effect inpatients with type 2 diabetes, we conducted this clinical trialto examine whether the supplement of ABM extract im-proves insulin resistance in patients with type 2 diabetes.
We also evaluated the adiponectin concentration using manyfasting serum glucose metabolic factors to further examinethe possible mechanism of its beneficial effects.
In this study, subjects who received the supplement of ABM extract showed a significant reduction in HOMA-IRindex (from 4.8 to 3.6), which was much lower than that ob-served among the control subjects (from 6.3 to 6.6).
It is interesting to note that the subjects who had taken a supplement of ABM extract for 12 weeks showed a signif-icant increase in plasma adiponectin concentrations com-pared with the placebo (cellulose) group (p Ͻ 0.001) (Fig.
1). Many previous studies have reported that the adiponectinlevel might be a major modulator of insulin resistance23 andpredict the development of type 2 diabetes.24–26 Circulatingadiponectin levels in human is positively correlated with in-sulin sensitivity.23,27 The level of adiponectin appeared to Change (%) in plasma adiponectin concentrations after play a very important role in the regulation of insulin act- 12-week treatment in Agaricus blazei Murill extract (ABME) groupand placebo (cellulose) group (p Ͻ 0.001). CI, confidence interval.
ing and energy homeostasis.27,28 We observed a 20% in-crease in adiponectin level after 12 weeks of treatment with AGARICUS BLAZEI SUPPLEMENT FOR INSULIN RESISTANCE
ABM extract supplement. This might account for the effect tion with ABM extract improves insulin resistance in type of improving insulin resistance in the ABM extract group.
2 diabetes. The increase in adiponectin concentration after Our finding reveals that after the 12-week treatment, sub- 12-week treatment with ABM extract supplement might be jects receiving ABM extract had a 6.7% reduction of HbA1C the mechanism that brings beneficial effect. Studies with a (from 8.9 to 8.3) and those taking cellulose had a 2.4% re- longer period of follow-up should be made in the future.
duction (from 9.1 to 8.9). Although both supplementsshowed a similar effect on reducing HbA1C with no statis-tical difference, the results still had clinical implications. Inthe AMB extract group, the decrease in HbA1C could be at- ACKNOWLEDGMENTS
tributed to the improved insulin resistance, whereas that inthe control group might be caused by loss of appetite or re- We thank all colleagues in Taipei Hospital, Taiwan for duced food intake. Although the initial results showed no helping with this study. This study was supported by grants statistical difference in HbA1C, treatment of longer dura- from the Taipei Hospital and Eng Chiao Bio-Technology tion might result in significant statistical difference.
As mentioned in our study design, all the subjects should maintain the same dose of gliclazide and metformin duringthe study period unless hypoglycemia occurs. During the 12- REFERENCES
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Ella Bay Development, Far North Queensland Expression of Interest in Conducting Research: Research Topic: Community marine safety: Placement of marine stinger enclosures and educating the community about venomous marine animals Research Team Carrette Matthew Gordon Avril Underwood Tropical Australian Background and research questions Then use of the marine environmen