USE OF THE HOMEOPATHY FOR THE IMPROVEMENT OF THE AMOUNT AND QUALITY OF EMBRYOS IN BOVINE (BRAZIL).
LOURENÇO, F.1 ; KOYAMA, R.2; LOPES, E.2
1 - Veterinary doctor - Veterinary Medicine School of the Paraná University. UNIPAR.
INTRODUCTION: The use of homeopathic products in the cattle industry has increased considerably in the last years. Among the several available products in the market, there are some destined to cows producing of embryos specifically with the premise of increasing not only the amount of viable structures, but also the quality of these embryos. OBJECTIVE: The objective of this experiment was to evaluate the effect of the use of homoeopathic products in cows donors of embryos to improve the amount and quality of the produced embryos. MATERIAL AND METHODS: A double blinded and randomized experiment was designed, using two (2) groups of nineteen (19) cows of the Indian race Brangus, allocated to brachiaria (Brachiaria brizhanta) pastures. The animals were allocated randomly to the two lots and the treatments were also chosen randomly and the lots named as CONTROL and TREATED groups. The animals of the TREATED LOT received three (3) homeopathic products: the CM&C FACTOR(*), the FERTIL FACTOR(*)and the STRESSES FACTOR(*), in the dosage of 2 grams of each product per animal a day and mixed in the mineral salt that was administered "ad libitum". The synchronization protocol that was used to provoke the superovulação consisted of the insertion of an intravaginal device saturated with 250 mg of medroxiprogesterona acetate and the application of 25 mg of progesterone base and 2 mg of estradiol benzoate on "Day 0", decreasing applications of FSH beginning on "Day 5 " and finishing on "Day 8." On "Day 7" it was administered 150mcg of prostaglandin F and 150 mcg in the afternoon. On “Day 8” the intravaginal device was removed in the morning, and all the animals had enter in heat on the “Day 9. These animals were inseminated with previously selected bulls' semen being accomplished 2 inseminations. The collections were accomplished seven days after the heat. The data related to the collections were registered in specific reports and submitted to statistical analysis. A total of 33 embryo collections were accomplished in three phases, always seven days after the heat. In the First Phase the collection was done in eight (8) cows, fifteen (15) in the Second Phase and ten (10) in the Third Phase. The collected embryos classified as Degree I and II were frozen. The embryos of Degree III were inoculated in cows of the farm (not for the embryos of the third collection) and the degenerate embryos and not fertilized were discarded. RESULTS: The homoeopathic treatment began after the first collection (Phase I) and it was administered during the second and third collections (Phases II and III).
Graph 1 - RECOVERED EMBRYOS
The total of collected embryos divided by the
number of animals in each phase were 18.13 for the
Phase I, of 20.87 for the Phase II and 20.10 for the
Phase III (Graph 1), what represented a significant
(p < 0.01) improvement of 15.5% and 11.0% in favor
Graph 2 – VIABLE EMBRYOS / DONOR - AVERAGE
The total of collected embryos divided by the
number of animals in each phase were 18.13 for
the Phase I, of 20.87 for the Phase II and 20.10 for
the Phase III (Graph 1), what represented a
significant (p < 0.01) improvement of 15.5% and
11.0% in favor of the homoeopathic treatment.
Graph 3 - DEGREE I EMBRYOS - AVERAGE PHASE III
The number of embryos classified as Degree I by
donor were 4.13 for the Phase I. of 5.13 for the Phase
II and of 8.10 for the Phase III (Graph 3) what
represents an improvement of 24.2% and of 50.1% in
favor of the homoeopathic treatment (p<0.05).
PHASE III Graph 4 - DEGENERATED EMBRYOS
The numbers of degenerated embryos per donor
were 9.75 at the Phase I, in the Phase II it was of
8.53 and 6.20 in the Phase III (Graph 4) what
represents a significant (p < 0.01) decrease of
12.5% and 36.4% respectively for the time using
PHASE III Graph 5 - DEGREE III EMBRYOS INNOCULATED(% OF POSITIVE GESTATION)
The collected embryos of degree II and III were
inoculated during the First and Second Phases in cows
of the breed Brangus and pregnancy diagnosis was
accomplished through rectal examination that showed
an index of pregnancy of 50.0% (9/18) and 81.8%
(9/11) respectively during the first and second phase,
what represents an increase of 63.6% (p < 0.05) after
CONCLUSIONS: The evaluation of the data demonstrated the effectiveness of the homeopathic treatment with the
products CM&C FACTOR , FERTIL FACTOR and STRESS FACTOR , for the improvement of the amount and quality of the embryos when using those products during the time of the collection of embryos and transfer period. .
(*) Trade Mark of the Veterinary Homeopathic Laboratory Fauna & Flora Arenales Ltd., Brazil.
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