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Chromatography, Vol.28 No.1 (2007) A Study on Data Analysis by Liquid Chromatography / Tandem Mass Spectrometry for the Determination of Veterinary Drugs in Livestock Foods Shigemi KAI, Takeshi AKABOSHI, Teruhisa FUJIMAKI and Shin−ichi ITO Kanagawa Prefectural Institute of Public Health, 1−3−1, Shimomachiya, Chigasaki−City, Kanagawa, Japan 253−0087 Received for review January 12, 2006. Accepted February 2, 2007. Abstract
The data analysis by liquid chromatography/tandem mass spectrometry (LC / MS / MS) for the determination of veterinary drugs in livestock foods was studied. LC / MS / MS acquisition parameters were established in positive and negative electrospray ionization (ESI) modes. In multiple reaction monitoring (MRM), target ions were used for the quantification, and relative intensities (intensity rations) of reference ion to target ion were used for the qualitative analysis. The relative intensities for reference are characteristic of veterinary drugs.
Keywords : liquid chromatography / tandem mass spectrometry, veterinary drug, livestock food Correspondence : Shigemi KAI, Chemistry Division, Kanagawa Prefectural Institute of Public Health, Shimomachiya 1−3−1,Chigasaki,
E−mail : [email protected]
Chromatography, Vol.28 No.1 (2007) Chromatography, Vol.28 No.1 (2007) Table 1. Operating conditions of LC/MS/MS for 34 veterinary drugs
Product ion, DP : Declustering Potential, CE : Collision Energy 5−propylsulfonyl−1H−benzimidazole−2−amine (ABZ−M) Chromatography, Vol.28 No.1 (2007) Figure 1. Typical LC/MS/MS MRM chromatograms of 13 sulfa
Figure 2. Typical LC/MS/MS MRM chromatograms of chicken
extract fortified at 0.01 µg/g of 13 sulfa drugs B : extract ion chromatogram m/z ; 281 B : extract ion chromatogram m/z ; 281 C : extract ion chromatogram m/z ; 281 C : extract ion chromatogram m/z ; 281 LC/MS/MS conditions as given in text.
LC/MS/MS conditions as given in text.
Chromatography, Vol.28 No.1 (2007) Figure 3. Typical LC/MS/MS MRM chromatograms of chicken
extractA : total ion chromatogram (TIC)B : extract ion chromatogram m/z ; 281 C : extract ion chromatogram m/z ; 281 LC/MS/MS conditions as given in text.
European Parliament Decision 2002/657/EC, Official Jour- nal of European Communities L 221, 8 (2002) Chromatography, Vol.28 No.1 (2007) Relative standard deviation (n=5) of peak area of veterinary drugs in solutions by LC/MS/MS 5−propylsulfonyl−1H−benzimidazole−2−amine (ABZ−M) Relative intensity on LC/MS/MS of reference ion to target ion with and without matrix (Swine, Chicken, Beef) (n=5) Relative intensity on LC/MS/MS of reference ion to target ion (n=5) *

Source: http://chromsoc.jp/Journal/pdf/28-1_29.pdf