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Nottingham Neonatal Service- Clinical Guidelines Guideline C2

Title: GENTAMICIN
Version:
2 (Vs 1 Aug 2004)
Ratification
Review Date:
Approval:
Nottingham Neonatal Service Clinical Guideline Meeting
Author: Dulip
Jayasinghe
Job Title:
Consultant Neonatologist
Consultation:
Nottingham Neonatal Service Staff and Clinical Guideline Meeting
Guideline
Dr Stephen Wardle, Guideline Coordinator and Consultant
Neonatologist co/ Stephanie Tyrrell, Nottingham Neonatal Service
[email protected]
Distribution:
Nottingham Neonatal Service, Neonatal Intensive Care Units,
Postnatal Wards.
Staff of the Nottingham Neonatal Service and Postnatal Wards
audience:
Patients to

Patients of the Nottingham Neonatal Service who fit the inclusion
whom this
criteria of the guideline below
applies:
Key Words:

Gentamicin, pre-dose, post-dose
Risk Managed: Sub optimal antibiotic dosing and Toxic drug levels
Evidence used: The contemporary evidence base has been used to develop this

guideline. References to studies utilised in the preparation of this
guideline are given at its end.

Clinical guidelines are guidelines only. The interpretation and application of clinical
guidelines remain the responsibility of the individual clinician. If in doubt, contact a

senior colleague. Caution is advised when using guidelines after the review date. This
guideline has been registered with the Nottingham University Hospitals NHS Trust.

Background

Gentamicin is an aminoglycoside antibiotic. It plays an important role in treating neonatal sepsis
and is used as a first line and second line antibiotic. It is bactericidal and active against some
Gram-positive and many Gram-negative organisms (broad spectrum). It is also active against
Pseudomonas aeruginosa. However it is inactive against anaerobes and is also inactive against all
streptococci and enterococci.
Pharmacology

Aminoglycosides are given systemically and excreted by the kidneys. There is little diffusion into
the CSF, even with inflamed meninges. Many of the side effects are dose related. Important side
effects include ototoxicity and renal impairment. If there is renal impairment, the dose interval
should be increased. In more severe renal impairment the dose should be reduced or the drug
avoided. Gentamicin should preferably not be given with ototoxic diuretics (furosemide). If
concurrent use is unavoidable, then they should be spaced apart as far as possible. Prolonged use
should be avoided. Gentamicin may impair neuromuscular transmission and must not be given in
myasthenia gravis.
Nottingham Neonatal Service- Clinical Guidelines Guideline C2

Dose

Post-menstrual Age
Frequency

NB. Post-menstrual age = Gestation at birth + post-natal age
Round doses down to the nearest 0.5mg.
Doses must be given as a slow bolus over 3-5 minutes

Levels

Take levels at the 3rd dose, the pre-dose immediately before the dose.
and the A post-dose level should only be measured when there is culture confirmation of an
organism that is sensitive to gentamicin or a senior member of the team feels it is necessary. The
sample should be taken, one hour after the dose.
If levels are adequate, they need only be checked twice weekly. If the levels are inadequate and the
dose is changed, recheck levels before and after the second new dose is given.
Desired levels


Renal Impairment

Gentamicin is often started on admission. If it becomes apparent that the baby has renal
impairment as part of his/her illness (poor urine output, rising creatinine, significant Hypotension)
then a trough level should be taken immediately before the 2nd dose is due. And withhold 2nd
dose until trough level is reported
.
In the presence of a significant renal diagnosis (baby requiring renal team involvement) then
Gentamicin is best avoided.
Adjusting Dosages

• If the trough is 2-3 micrograms/ml ⇒ Increase the interval between doses by 12 hours and • If the trough is >3 micrograms/ml ⇒ withhold next dose, discuss with senior staff, repeat trough 12 hours later and review renal function •If the peak is >10 micrograms/ml ⇒ Decrease dose by 10-20% • If the peak is <5 micrograms/ml ⇒ Increase dose by 10-20%
• If the peak is 5-7 micrograms/ml in the presence of severe infection (incl
pseudomonas)⇒ Discuss with senior staff and consider increasing the dose by 10%

And Recheck levels at the next dose after the changed dose is given
Nottingham Neonatal Service- Clinical Guidelines Guideline C2


Elevated gentamicin levels and hearing

Aminoglycoside use is associated with later high frequency hearing loss but the relationship is
unclear. The background rate of newborn sensorineural deafness is 1/1000. The most common
risk factors is admission to NICU for >48 hours, a FH of early childhood permanent deafness or
craniofacial abnormality, but in half of deaf children no risk factor is identified.
If the pre- or post-dose level is elevated then the parents should be informed of this and that the
baby will receive an appointment for a hearing test at 8 months. The local hearing screening
manager receives details of all infants with elevated gentamicin levels and informs the Childrens
hearing assessment centre.
Processing Levels

Prescribe the initial 3 doses of Gentamicin.
Review Culture results, Clinical history and Current Clinical status at 48 hours and decide if more
than 2 doses are necessary. If the course continues, proceed to level measurements ‘around’ the
3rd dose.
Specific arrangements for NCH
Gentamicin levels go from the fridge at 9am.
Other samples could be sent at 12 midday on Mondays-Fridays to catch the 1pm shuttle to the
QMC, from the pathology laboratory reception (the porters have kindly agreed to do another regular
collection at 12.00 midday Monday to Friday).
At weekends the doctors need to remember to phone the microbiology doctor [not technician] for
results about 143.00 if they have not already been sent by phone.
Interactions

Be aware of the increased risk of ototoxicity and nephrotoxicity with
Cephalosporins
Vancomycin
Furosemide
Amphotericin
Indomethacin
References

Nottingham Neonatal Service- Clinical Guidelines Guideline C2

APPENDIX 1
Pre- and post- dose levels Gentamicin Prescribing Chart
Gentamicin Prescribing (1 per course)

of Dose Prescribed Checked Given Batch Level Pharm Prescribe initial course of 3 doses and mark dates and times, only, of the fourth
dose Prescribe the subsequent doses after levels are obtained
/ /

Initial Prescribing
Prescribe the first 3 doses only and clearly indicate the timing of a level assay. Add
Gentamicin to the treatment card and write ‘see attached sheet’. Staple this sheet to the
current drug chart. Levels are done just before and 1 hour after a dose.
Level Monitoring Table
Date Time Level Result due

Monitoring Guidelines
Do levels around the 3rd dose of Gentamicin when first starting this drug
Do levels around the 2nd dose if there are concerns about renal failure
Do levels around the 2nd dose of Gentamicin after any dose changes
Nottingham Neonatal Service- Clinical Guidelines Guideline C2

APPENDIX 2
The guideline below (No. 12.8) was removed from use as an independent guideline when the
guideline above (2.7, now renamed C2) was circulated in December 2004. It is to remain as
an appendix to guideline C2.

Nottingham Neonatal Service – Clinical Guidelines

Title:
Pre- and Post-Gentamicin Levels
Final Version 3.
August 2000. (Version 1: February 1995, Version 2 (reformatted only):
March 2000)

August 2003
Reviewed by Ruth Moore, Neonatal Nurse Educator. Version 1: Tracey
Mackay, Staff Nurse. Version 2/Reformat: Ruth Moore, NNE

Version 1: Microbiologists, CHN & UHN. Version 2: Neonatal medical &
nursing staff + Pharmacist (QMC) via Policy Meeting March 2000.

Neonatal Units
Reduce risk of side effects due to Gentamicin toxicity

Clinical guidelines are guidelines only. The interpretation and application of clinical
guidelines will remain the responsibility of the individual clinician. If in doubt contact a
senior colleague. Caution is advised when using guidelines after a review date. This
guideline has been registered with the Nottingham City Hospital Trust.


1. Introduction/Background
Gentamicin is active against Gram positive or Gram negative organisms, especially Proteus
organisms. Enterococci are often resistant.
It is used for urinary tract, pulmonary and miscellaneous infections, septicaemia and burns. For the
recommended dose and monitoring times, refer to the Nottingham Neonatal Service Antibiotics
Dosage Guide 1991, Policy No. 2.2(1)
2. Patient
Group/Indications
Gentamicin toxicity is essentially limited to loss of labrinthine function by degeneration of vestibular
sensory cells. It can also be nephrotoxic. Serum levels at which this occurs cannot be defined(2).
Levels need to be assayed around the 4th dose. Pre-dose level to be taken 5 minutes before the
dose and the post-dose level to be taken 1 hour after the dose.
3. Identification/Diagnosis
Pre- and post-gentamicin levels may be taken by the medical staff, ANNPs or a nurse who has
expanded their role and completed the relevant documentation(3)
4. Management
4.1

General patient management

List of equipment required
1) 2 plain clotting sample bottles (clear, conical shape)
2) Autolets, lancet, platforms
3) Gloves
Nottingham Neonatal Service- Clinical Guidelines Guideline C2

printed label X 1 per form plain labels or patient identification labels x 2 for bottles • Confirm when the level is due, this will be indicated by a box drawn around the time by the • Wash hands thoroughly and put on gloves(4) • Take sample of blood from the baby’s heel as per Nottingham Neonatal Service Clinical • Fill the bottle to the level 0.5 –1 ml blood • Ensure bleeding has stopped and, if necessary, apply a plaster. • Label bottle with child’s name, unit number, date of birth and time of sample • Administer Gentamicin following the Nottingham Neonatal Service Clinical Guideline 4.12(6) • Take post-gentamicin level 1 hour following administration of gentamicin, following all the same • Complete the printed label and attach to the microbiology form ensuring the following details are included: current gestation and weight; dose of gentamicin and start date of this course; exact time pre- and post-dose levels taken; urea results; and any other antibiotics the baby is receiving. • Write baby’s name in results book. State that gentamicin levels checked and inform medical staff/ANNP. Document on fluid chart that level checked.
4.2 Specific
• Gentamicin levels should only be performed by capillary blood sampling if an arterial line is not • If an arterial line is present, the gentamicin levels should be performed by medical staff/ANNP. • It will remain the responsibility of whoever is prescribing the gentamicin, to indicate when levels are required, by placing a box on the appropriate time on the prescription chart. • It will remain the medical staff’s/ANNP’s responsibility to act on the result, but the nurse should inform them that a level has been checked. • Completed incident forms relating to gentamicin levels • Audit of this expanded role. Scope of Professional Practice.
6. Allied
Guidelines/References
1. Nottingham Neonatal Service Clinical Guideline 2.2, 1991, Therapeutic Guideline sin neonatal Infection and Neonatal Antibiotics Dosage Guide. Dr. Isphani/Dr McLain 2. Wood, BSB, Insley, J, 1990. Paediatric Vade Mecum. 3. Nottingham Neonatal Service Scope of Professional Practice Framework: Expanded Clinical 4. Nottingham Neonatal Service Clinical Guideline 5.11. The wearing of gloves on neonatal units, 5. Nottingham Neonatal Service Clinical Guideline 12.6. Procedure for obtaining a capillary blood sample for glucose monitoring using an Autolet finger-pricking device. 1991. 6. Nottingham Neonatal Service Clinical Guideline 4.12. Administration of intravenous drugs and

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