Technical data and operating instructions Vivaspin® Turbo 15 – Introduction Storage conditions|shelf life Equipment Required For use with centrifuge
1. Centrifuge with swing bucket or fixed
Introduction
Vivaspin® Turbo 15 centrifugal concentrators
offer the optimal solution to any concentra-
Carrier Required
tion or buffer exchange application with
Highest flow rates are achieved due to their
double verticle membranes which minimize
protein polarization and subsequent fouling
of the membrane. Additionally, their sleek
internal profile ensures maximum process
speeds right down to the last 150 µl.The UV
joining technology allows for a smooth joint
housing, allowing complete sample recovery
from the unique pipette friendly deadstop
pocket. The dead stop pocket (patent pend-
ing) is an impermeable concentrate pocket
surface, thereby eliminating the risk of filtra-
The Vivaspin® Turbo 15 devices are the opti-
mal solution for protein concentration from
dilute samples e.g. after chromatography,
cell culture supernatants or fermentation
Operation
Vivaspin® Turbo 15 concentrators can be
used in swing bucket or fixed angle rotors
accepting standard conical bottom tubes. In
a single spin, solutions can be concentrated
up to 150 +. Samples are typically concen-
Equipment Required Equipment required Vivaspin® Turbo 15 Centrifuge Rotor type Rotor compatibility
Please note: Vivaspin® Turbo 15 (30 mm +
118 mm) is designed to fit into rotors that
with TS-5.1-500 swing-out rotor with BUC
356966 adaptors; Heraeus Multifuge 3 S-R
with (Heraeus/Sorvall) 75006445 swing out
rotor with 75006441 buckets and adaptors
for Falcon 50 ml conical bottom tubes.
These devices are not designed to fit into
please use the Vivaspin® 15, which can be
Operation Desalting | Buffer Exchange
1. Concentrate sample to desired level.
recovery select a MWCO at least 50% smaller
than the molecular size of the species of
3. Refill concentrator with an appropriate
4. Concentrate the sample again and repeat
contaminating microsolute is sufficiently
reduced. Typically, 3 wash cycles will remove
used, angle concentrator so that the printed
in table 2, taking care not to exceed the
achieved, (see table 3a and 3b for guide
to concentration times), remove assembly
Technical Specifications Table 1: Technical Specifications Vivaspin® Turbo 15 Dimensions Total Length Concentrator Capacity Swing bucket rotor Materials of construction Concentrator insert Table 2: Recommended Spin Speed (xg) Vivaspin® Turbo 15 Centrifuge
* Dead stop volume as designed in moulding tool. This volume may vary depending on
sample, sample concentration, operation temperature and centrifuge rotor. Usage Tips 1. Flow Rate 3. Sterilisation of Polyethersulfone
Filtration rate is affected by several para-
Membranes
meters, including MWCO, porosity, sample
concentration, viscosity, centrifugal force
and temperature. Expect significantly longer
spin times for starting solutions with over
To sterilise, use a 70% ethanol solution or
5% solids. When operating at 4°C. flow rates
4. Chemical Compatibility
glycerine will take up to 5 times longer to
Vivaspin® concentrators are designed for use
concentrate than samples in a predominantly with biological fluids and aqueous solutions.
For chemical compatibility details, refer to
2. Pre-rinsing
Membranes fitted to Vivaspin® concentrators
Sodium azide. Should these interfere with
analysis they can be removed by rinsing fill
volume of buffer solution or deionised water
through the concentrator. Decant filtrate
and concentrate before processing sample
pre-rinsed device immediately, store it in the
refrigerator with buffer or water covering
the membrane surface. Please do not allow
Performance Characteristics Table 3: Performance Characteristics Vivaspin® Turbo™ 15 Time to concentrate up to 20x [min.] at 20°C and solute recovery % Rotor Chemical Compatibility Table 4: Chemical Compatibility (2hr contact time) Solutions Solutions Compatible pH range pH 1–14 Compatible pH range pH 1–14
OK = Acceptable ? = Questionable NO = Not recommended
Ordering Information Qty per box Prod. no. Other Products Sample volume Membranes available
37079 Goettingen, GermanyPhone +49.551.308.0
been bleached without any use of chlorine
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Technical data and operating instructions